Vol 8, No 5 (2011)

Background. to comparatively investigate cytokine gene expression in the skin and peripheral blood of atopic dermatitis (AD) patients and healthy individuals. Methods. Samples of skin and peripheral blood from 48 severe AD patients SCORAD (Scoring Atopic Dermatitis) 78,5 [57; 89], IGA (Investigators Global Assessment) 4,2 [3,9; 4,7]) at the age of 17 to 45 years and 20 healthy donors aged from 19 to 32 years were analyzed for gene expression of cytokines using real time reverse transcription polymerase chain reaction (RT-PCR). Results. In the skin of patients with AD, a significant increase of the level of gene expression was observed for interleukin IL2R (interleukin) (р=0,0023), IL5 (р=0,002), IL6 (р=0,0023), IL8 (р=0,01), IL12β (р=0,0023), IL10 (р=0,0023), IL23 (р=0,002), IL29 (р=0,0023), and TGFβ (transforming growth factor) (p=0,0023) as compared to healthy individuals. In contrast, no difference between AD patients and healthy donors was detected with respect to cytokine gene expression in the peripheral blood. Conclusions. Activity of IL-2R, IL-8, IL-12β, IL-23, IL-29, and TGFβ that are markers of chronic inflammation and Th1 immune response in severe AD and IL-5, IL-10 that are anti-inflammatory cytokines and markers of Th2 response was predominant in the skin but not in the blood of AD patients.

Background. To investigate the expression of surface molecules CD4+CD25high and CD4+FoxP3+ on regulatory T-cells (Treg) in patients with different severity of bronchial asthma (BA). Methods. We included 19 patients with severe BA, 12 patients with mild BA and 17 healthy donors. The circulating percentage of CD4+CD25high and CD4+FoxP3+ T-reg in peripheral blood was estimated by the flow cytometry analysis (FACSCalibur, Becton Dickinson, USA). Results. Numbers of peripheral blood CD4+CD25high was significantly decreased in mild BA compared to healthy control. Patients with severe BA had increased levels of CD4+FoxP3+ and CD4+CD25high compared to mild BA. Conclusion. Our results demonstrate new scientific data on the phenotypic characteristics and the level of T-reg in peripheral blood at different clinical forms of asthma. This fact confirms the existence of immunoregulatory control mechanisms of allergic inflammation involving T-reg.

Background. The research is focused on the particularities of formation and course of atopic dermatitis in infancy depending on the range of sensitization and class of revealed antibodies. Methods. The article presents the results of clinical and immunological observation of 116 children aged from 1 month to 3 years with the clinical-anamnestic signs of atopic dermatitis. The immunological observation consisted of the following stages: determination of concentration of the general IgE, quantitative testing of concentrations of the serum IgE and IgG4 antibodies to allergens of cow and goat milk, chicken eggs and wheat, house dust and through the immune-enzyme analysis. Results and discussion. According to the results of the immunological observation the patients were divided into 3 groups: I - IgE+IgG4 - positive, II - IgG4 - positive, III - absence of above-mentioned classes of antibodies. The patients of group I are characterized by the early sensitization to cow milk proteins, the manifestation of the disease up to 3 months appears on pure breast feeding. The detection of the specific IgE antibodies to allergens of chicken eggs is a sign of danger of the development of sensitization towards inhalation house dust allergens and of the formation of respiratory allergy. For the children of group II the later age of manifestation of skin process (in more than half of children after introduction of milk food) and easier course of disease are significant. Anamnesis of the disease and the clinical manifestations of one third of patients of group III in the absence of the evidence of IgE-sensitization coincides with that of the patients of group I (age of manifestation, severity of disease). In accordance with received results, we can suggest that in this group there are patients with the unidentified immunopathological mechanism of formation of the disease. Conclusion. The immunological observation of infants by the detection of the specific IgE and IgG4 antibodies to widespread food allergens allows to reveal the peculiarities of immune response which in their turn define the clinical-anamnestic signs of atopic dermatitis.

Cow's milk allergy (CM A) is the most common food allergy in young children. Babies who develop cow's milk protein (CMP ) allergy may have one or several of the following symptoms: skin rash or gastrointestinal symptoms (abdominal pain or cramps, vomiting or diarrhea). The symptoms can be controlled through a dairy-free diet. So the diagnosis and elimination of CMP is an essential element in management of children with CM A. This article provides a summary of extensive clinical research behind the Nutrilon Aminoacid, demonstrating the clinical efficacy through 25 years of experience.

The article is devoted to modern approaches of H1-antihistamines for the treatment of children with allergic disease.

In this article the case of AD patient with secondary infertility is presented. The therapy of this patient included the course of modern hepatoprotector and immunomodulator «Laennek» («Japan Bioproducts Industry Co has been spent., Ltd», Japan), which is a human placenta hydrolysate. The treatment has allowed not only to reach AD remission, but also has influenced a current of secondary infertility and has led to long¬expected pregnancy.