Vol 10, No 5 (2013)

Background.. To investigate expression of cytokines genes parameters in skin and blood in severe atopic dermatitis (AD) and cutaneous T-cell lymphoma (CTCL) patients comparing with healthy donors. Materials and methods. 20 severe AD patients, 20 CTCL patients and 20 healthy donors were included in the study. Skin samples and peripheral blood were used as material for immunological study. Interleukins — (IL)1B, IL2, IL2r, IL4, IL5, IL6, IL7, IL8, IL10, IL12A, IL12B, IL15 (total), IL15 , IL17A, IL18, IL23, IL28, IL29, Interferon γ (IFNγ), tumor necrosis factor α (TNFα), transforming growth factor beta 1 (TGFB1), forkhead box P3 (FOXP3) gene expression was defined in the skin and peripheral blood of severe AD patients, CTCL patients and healthy donors by real-time reverse transcription polymerase chain reaction (RT-PCR). Results. Statistically significant increase of cytokines genes IL4,IL12B,IL17A, TNFα in peripheral blood of severe AD patients compared with CTCL patients was marked. Studying of skin samples from CTCL patients has shown statistically significant increase of cytokines IL8, IL10, IL15, IFNγ genes expression and decrease of IL18 gene expression in comparison with skin samples from severe AD patients. Conclusion. Obtained cytokines genes expression cytokines genes parameters in peripheral blood of severe AD and CTCL patients had a certain similarities consisting of increased IL8, IFNγ and decreased IL6, IL23 genes expression in comparison with healthy donors. Substantial differences in peripheral blood of patients in comparison with healthy donors: increased IL-5, IL-12B genes expression in AD patients and decreased IL-8, IL-12A genes expression in CTCL patients, at the same time increased expression of IL-6, IL-8, IL-10, IFNγ genes in severe AD and CTCL patients were shown.

Recommendations contain modern data about incidence, etiology, patogenesis, diagnostics and treatment of drug allergy.

This article describes the features of asthma phenotypes such as asthma in smokers and asthma and obesity. The role of cysteinyl leukotrienes is discussed in the formation of inflammation in these phenotypes of asthma. The leukotriene receptor antagonists, including montelukast are effective in therapy of this category of patients.

The prevalence of herpes simplex virus in different age groups, the high frequency of relapsing forms of infection, torpid course, postherpetic complications are relevant medical and social problems. Often, practitioners create significant difficulties in establishing the diagnosis, choice of strategy in patients with herpes simplex. The paper presents the methods of diagnosis of herpes simplex and their application in atopic dermatitis patients, issues of acyclic nucleoside therapy (acyclovir, famciclovir) in different clinical forms of herpes simplex with individual approach to the selection of an antiviral drug and patterns of use.