LOCAL AND SYSTEMIC IMMUNE PARAMETERS IN SEVERE ATOPIC DERMATITIS AND CUTANEOUS T-CELL LYMPHOMA PATIENTS



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Abstract

Background.. To investigate expression of cytokines genes parameters in skin and blood in severe atopic dermatitis (AD) and cutaneous T-cell lymphoma (CTCL) patients comparing with healthy donors. Materials and methods. 20 severe AD patients, 20 CTCL patients and 20 healthy donors were included in the study. Skin samples and peripheral blood were used as material for immunological study. Interleukins — (IL)1B, IL2, IL2r, IL4, IL5, IL6, IL7, IL8, IL10, IL12A, IL12B, IL15 (total), IL15 , IL17A, IL18, IL23, IL28, IL29, Interferon γ (IFNγ), tumor necrosis factor α (TNFα), transforming growth factor beta 1 (TGFB1), forkhead box P3 (FOXP3) gene expression was defined in the skin and peripheral blood of severe AD patients, CTCL patients and healthy donors by real-time reverse transcription polymerase chain reaction (RT-PCR). Results. Statistically significant increase of cytokines genes IL4,IL12B,IL17A, TNFα in peripheral blood of severe AD patients compared with CTCL patients was marked. Studying of skin samples from CTCL patients has shown statistically significant increase of cytokines IL8, IL10, IL15, IFNγ genes expression and decrease of IL18 gene expression in comparison with skin samples from severe AD patients. Conclusion. Obtained cytokines genes expression cytokines genes parameters in peripheral blood of severe AD and CTCL patients had a certain similarities consisting of increased IL8, IFNγ and decreased IL6, IL23 genes expression in comparison with healthy donors. Substantial differences in peripheral blood of patients in comparison with healthy donors: increased IL-5, IL-12B genes expression in AD patients and decreased IL-8, IL-12A genes expression in CTCL patients, at the same time increased expression of IL-6, IL-8, IL-10, IFNγ genes in severe AD and CTCL patients were shown.

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About the authors

D D Niyazov

Institute of Immunology

Email: daniyarniyazov@yandex.ru
Moscow, Russia

E S Fedenko

Institute of Immunology

Moscow, Russia

M N Boldyreva

Institute of Immunology

Moscow, Russia

D Yu Trofimov

Institute of Immunology

Moscow, Russia

References

  1. Тарасенко Ю.Г. Клиническая и морфоиммуногистохимическая дифференциальная диагностика лимфом кожи. Автореферат диссертации канд. мед. наук. М., 2008, 22 с.
  2. Лезвинская Е.М., Овсянникова Г.В. Особенности диагностики различных нозологических форм злокачественных лимфом кожи. Дерматовенерология. 2009, № 1, с. 110-113.
  3. Sokolowska M.W., Baranska W., Cegielska A. Atopic dermatitis-like Pre-Sezarysyndrome: Role of Immunosupression. Actaderm. Venereol. 2011, v. 91, p. 574-577.
  4. Arellano F.M., Arana A., Wentworth C.E. et al. Lymphoma among patients with atopic dermatitis and/or treated with topical immunosuppressants in the United Kingdom. J. Allergy Clin. Immunol. 2009. v. 123 (5), p. 1111-1116.
  5. Cooper K.D. Atopic Dermatitis: recent trends in pathogenesis and therapy. J. Invest. Dermatol. 2003, v. 102, p. 128-137.
  6. Maintz L., Novak N. Getting and more complex: the pathophysiology of atopic eczema. Eur. J. Dermatol. 2007, v. 17 (4), p. 267-283.
  7. Лезвинская Е.М. Злокачественные лимфомы кожи. М., «Практическая медицина». 2010, 358 с.
  8. Hanafusa T., Matsui S., Murota H. et al. Increased frequency of skin-infiltrating FoxP3(+) regulatory T-cells as a diagnostic indicator of severe atopic dermatitis from cutaneous T-cell lymphoma. Clin. Exp. Immunol. 2013, v. 172 (3), p. 507-512.
  9. Meyer N., Mazereeuw-Hautier J., Launay F. et al. Cutaneous T-cell lymphoma complicating severe atopic dermatitis. Ismakingadiagnosisthemainchallenge? Dermatology. 2009, v. 218 (2), p. 168-171.
  10. Российский национальный согласительный документ по атопическому дерматиту. Под ред. Р.М. Хаитова, А.А. Кубановой. Атопический дерматит: рекомендации для практических врачей. М., «Фармарус Принт». 2002, 24 с.
  11. Ребриков Д.В., Саматов Г.А., Трофимов Д.Ю. и соавт. ПЦР в «реальном времени». М., БИНОМ. Лаборатория знаний. 2009, 115 с.
  12. Wismer J.M., McKenzie R.C., Sauder D.N. Interleukin-8 immunoreactivity in epidermis of cutaneous T-cell lymphoma patients. Lymphokine Cytokine Res. 1998, v. 13 (1), p. 21-27.
  13. Leroy S., Dubois S., Tenaud I. et al. Interleukin-15 expression in cutaneous T-cell lymphoma (mycosis fungoides and Sézary syndrome). Br. J. Dermatol. 2001, v. 144 (5), p. 1016-1023.
  14. Asadullah K., Haeussler-Quade A., Gellrich S. et al. IL-15 and IL-16 overexpression in cutaneous T-cell lymphomas: stage-dependent increase in mycosis fungoides progression. Exp. Dermatol. 2000, v. 9 (4), p. 248-251.
  15. Ярилин А.А. Иммунология. М., «ГЭОТАР-Медиа». 2010, 752 с.
  16. Trzeciak M., Glen J., Bandurski T. et al. Relationship between serum levels of interleukin-18, IgE and disease severity in patients with atopic dermatitis. Clin. Exp. Dermatol. 2011, v. 36 (7), p. 728-732.
  17. Gilbert L.A., Hemann M.T Context-specific roles for paracrine IL-6 in lymphoma. Genes Dev. 2012, v. 26, p. 1758-1768.
  18. Grewe M., Bruijnzeel-Koomen C.A., Schopf E. et al. A role for Th1- and Th2-cells in the immunopathogenesis of atopic dermatitis. Immunol. Today. 1998, v. 19, p. 359-361.

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