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Vol 9, No 6 (2012)

Articles
Editorial
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Russian Journal of Allergy. 2012;9(6):3-5
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Pharmacogenetics of uncontrolled asthma
Kulikov E.S., Ogorodova L.M., Freidin M.B., Deev I.A., Rudko A.A., Selivanova P.A.
Abstract
This review summarizes the results of pharmacogenetic studies of determination response to drugs used for the treatment of bronchial asthma. Pharmacogenetic information will enable the use of a personalized approach to the asthma management, which will adjust the therapy technology and increase the possibility of achieving disease control.
Russian Journal of Allergy. 2012;9(6):6-14
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TREATMENT OF URTICARIA OFF-LABEL
Danilycheva I.V., Pecherey I.O.
Abstract
The application of the drug, as indicated in the dosage form, dosage regimen, in population or other application parameters that are not mentioned in the approved instructions, called the use of off-label treatment. The prevalence of this phenomenon is as high as 90% in some areas. The vast majority of drugs used for the treatment of chronic urticaria, have no indication for its treatment. To reduce the risk of drug administration off-lable it is necessary to evaluate the clinical situation, the quality of evidence for the efficacy and safety of the drug. There are three categories of situations to make a decision to use off-lable treatment: to use justifiable proof of the quality, to use in the context of scientific research, to use in exceptional cases justified by special clinical situations. Legal support can help the doctor to decide responsibly to treat a patient offlabel.
Russian Journal of Allergy. 2012;9(6):15-23
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SIRNA-MEDIATED INHIBITION OF INTERLEUKINE-13 PRODUCTION IN VITRO
Shilovskiy I.P., Mazurov D.V., Shershakova N.N., Khaitov M.R.
Abstract
Background. According to current views, one of the major mediators involved in the development of allergic process is IL-13. The goal of this work was to design small interfering RNA molecules to effectively inhibit il-13 gene expression of mice in experiments in vitro. Methods. For the expression of IL-13 in in vitro gene coding sequence il-13 was amplified using cDNA ConA-stimulated spleen cells from BALE / c mice as a template and cloned into the expression vector pUCHR IRES GFP. Using a computer analysis were designed six variants of siRNA, directed against mRNA-il-13. To test the efficiency of siRNA a co-transfection of 1x 105 cells HEK293T mixture (0,5 mg and 1 mg of plasmid siRNA) coupled with Lipofectamine 2000 reagent was carried out. Twenty-four hours later, the gene expression changes in il-13 recorded by flow cytometry on the fluorescence intensity of GFP+-cells. Gene expression of il-13 mRNA was assessed by quantitative PCR, and the level of the protein product by ELISA. results. As a result, siRNA molecules were obtained and three of them were able to effectively inhibit the gene expression of il-13. Conclusion. Thereby variants of siRNA, which can effectively inhibit the production of mice’s IL-13 in vitro; can be used later in experiments in vivo so to understand the role of IL-13 in the pathogenesis of allergic conditions as to develop new therapy approaches.
Russian Journal of Allergy. 2012;9(6):24-27
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PRODUCTION AND CHARACTERIZATION OF RECOMBINANT PROTEINS CONTAINING BET V 2 ANTIGENIC EPITOPES OF BETULA PENDULA
Chernysheva A.V., Tyutyaeva V.V., Pivovarova A.V., Andreev I.V., Sankov M.N., Kuzmenko Y.V., Starodubova E.S., Martynov A.I., Karpov V.L.
Abstract
Aim of investigation. Production of immunologically active recombinant protein of Bet v 2 allergen ofbirch pollen. Materials and methods. mRNA was isolated from a sample ofbirch pollen. cDNA library was derived using SMART technology. Gene Bet v 2 was amplified by means of PCR with primers from the cDNA. The resulting PCR fragment of the gene was cloned into the vector pET29b(+). The recombinant protein Bet v 2 was expressed in cells E. coli, transformed with a plasmid. The recombinant protein was purified using NiNTA agarose. Immunological activity of the recombinant protein Bet v 2 was measured by ELISA and immunoblot methods. Results. The production system of the recombinant allergen Bet v 2 preparation suitable for immunological tests was developed during the research project. In the first phase allergen Bet v 2 gene was cloned from birch pollen collected in Russia. The gene was inserted into the vector pET29(+) for expression in bacterial cells. The expression cell strain E. coli was obtained with this plasmid. The synthesis of the recombinant protein that accumulates in inclusion bodies was activated in bacterial cells. The procedure of recombinant protein Bet v 2 isolation from inclusion bodies was developed by one round of chromatography purification. The recombinant protein isolation was carried out by chromatography on Niagarose. The highly purified preparation of the recombinant allergen was obtained as a result. The recognition of the recombinant protein Bet v 2 by sera varied in ELISA, indicating a different degree of patients sensitization to this allergen. In the immunoblot test the preparation was active only in 15% of cases. Apparently, reactive epitopes of the allergen are mainly conformational ones and are active in ELISA test, whereas linear epitopes, that are active in immunoblot, are in the minority. Conclusion.The system for production of recombinant allergen Bet v 2 preparation suitable for immunological tests has been developed.
Russian Journal of Allergy. 2012;9(6):28-31
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TACROLIMUS IN TREATMENT OF ATOPIC DERMATITIS PATIENTS WITH SIDE EFFECTS OF LONG LASTING TOPICAL CORTICOSTEROIDS APPLICATION. CASE REPORTS
Elisyutina O.G., Fedenko E.S., Shtirbul O.V.
Abstract
The own data of the retrospective analysis of 88 atopic dermatitis (AD) clinical cases from 2011 to 2012 are presented in this article. 3 clinical cases of tacrolimus using in AD patients, who had side effects of long lasting topical corticosteroids application are described.
Russian Journal of Allergy. 2012;9(6):32-38
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INFLUENCE OF THE FUNGAL MICROFLORA ON THE COURSE OF PERENNIAL ALLERGIC RHINITIS IN CHILDREN
Malanicheva T.G., Akhmadieva L.F., Denisova S.N.
Abstract
Background. The purpose of our research was to study the influence of fungal microflora for perennial rhinitis in children to improve the treatment of this disease. Methods. 79 children aged from 7 to 18 years with perennial allergic rhinitis (PAR) with fungal colonization of the nasal mucosa were examined. Patients underwent clinical, allergy, culturing mycological examination of the mucous membranes of the nose, as well as determination of circulating Candida antigen in serum by ampero-metric enzyme immunoassay sensor. Results. Fungi Candida were dominated — in 75,9% of cases in the structure of mycotic colonization, while mold fungi Aspergillus were observed rarely (24,1%). Colonization of the nasal mucosa with fungi Candida in children with PAR makes disease severe and symptoms become more painful that manifest severe nasal congestion, abundant mucus. PAR patients with nasal colonization of the fungi genus Candida in 58,3% of cases in the circulating blood serum had Candida antigen, that indicating the development of invasive Candida infections. That's why therapy of this disease must be improved. Inclusion of antimicrobial agents to the treatment with further traditional therapy of PAR leads to an overall therapeutic effect in 77,1% of cases. Conclusion. In patients with PAR fungal microflora aggravates the disease and the treatment needs to be improved with the inclusion of antibiotics, which lead to high clinical improvement.
Russian Journal of Allergy. 2012;9(6):39-43
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SIDE-EFFECTS AND PATIENTS COMPLIANCE OF SUBCUTANEOUS ALLERGEN-SPECIFIC IMMUNOTHERAPY WITH BIRCHPOLLEN EXTRACT ABSORBED ON CALCIUM PHOSPHAT
Shakhova N.V., Gordeev V.V., Bishevskaya N.K., Ardatova T.S., Tokarev V.P., Kharchenko S.S.
Abstract
Subcutaneous allergenspecific immunotherapy weakens the symptoms of allergic rhinoconjunctivitis and bronchial asthma. However, there exists the risk of local reactions emergence, as well as the threat of the dangerous anaphylactic reactions. Background. The purpose of this work is to evaluate the frequency and severity of the side-effects and to assess the patients’ compliance with subcutaneous allergenspecific immunotherapy, which uses the tree pollen allergen absorbed onto the calcium phosphate suspension. Methods. The study included 21 children at the age from 5 to 17 with allergic rhinoconjunctivitis with the sensibilization to the allergens of the tree pollen (birch, alder, hazel). The patients had been receiving the treatment with the tree pollen allergen absorbed onto the calcium phosphates suspension for 2 years. All cases of systemic and local side-effects, which appeared during the study, have been analised. Results. In 86% of cases (18 out of 21 patients) local reactions were found. During the study, the total number of injections was 740 with 169 cases of local reactions, which constitutes 22,6% of all injections. In 14,3 percent of cases only slight reactions have appeared. However, 8,3% of injections resulted in pronounced reactions. No systemic reactions were observed; neither of patients has refused to get treatment due to the side-effects. Conclusion. The results of the study have shown the high level of compliance and safety of the subcutaneous allergenspecific immunotherapy, which is based on the use of the tree pollen allergen absorbed onto the calcium phosphate suspension.
Russian Journal of Allergy. 2012;9(6):44-48
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SEVERE ASTHMA: PHENOTYPES AND ROLE OF SMALL AIRWAYS
Fassakhov R.S.
Abstract
The role of small airways in severe asthma is discussed. The involvement of small airways in the inflammatory process increases the importance of the value delivery systems for effective anti-inflammatory therapy. Extrafine aerosol beclomethasone dipropionate / formoterol (Foster) delivers the drug in the small bronchi significantly improving patient outcomes.
Russian Journal of Allergy. 2012;9(6):49-54
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TOPICAL TREATMENT OF ATOPIC DERMATITIS COMPLICATED WITH MICROBIAL INFECTION IN CHILDREN
Revyakina V.A.
Abstract
The role of microbial flora and topical treatment in atopic dermatitis in children is discussed.
Russian Journal of Allergy. 2012;9(6):55-59
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REGULATORY ROLE OF INTERLEUKIN-1 IN LOCAL INFLAMMATION AND TISSUE REGENERATION IN SKIN WOUNDS MODEL
Varyushina E.A., Antsiferova M.A., Alexandrov G.V., Sazonova T.A., Gudima G.O., Simbirtsev A.S.
Russian Journal of Allergy. 2012;9(6):62-63
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SPECIFIC METHODS OF ALLERGIC DISEASES THERAPY IN SPORTSMEN
Shartanova N.V.
Russian Journal of Allergy. 2012;9(6):64-65
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FEATURES OF NASAL MUCOCILIARY ACTIVITY IN REFRACTORY SINUSITIS
Khirnetkina A.F., Pavlova K.S., Evseeva V.V., Nosulya E.V., Kurbacheva O.M.
Russian Journal of Allergy. 2012;9(6):66-67
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Trends in controller treatment of patients with asthma IN primary health care facilities and asthma control (1998-2011) in saint-petersburg, russia
Emelyanov A.V., Tsukanova I.V., Fedoseev G.B., Sergeeva G.R., Lisitsyna N.F., Bakanina L.A., Nikitina E.Y.
Russian Journal of Allergy. 2012;9(6):68-69
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PERSPECTIVES OF APPLICATION OF RECOMBINANT HUMAN INTERLEUKIN-1 BETA IN TREATMENT OF ACUTE DAMAGES OF STOMACH MUCOSA IN RATS
Sazonova T.A., Varyushina E.A., Aleksandrov G.V., Gudima G.O., Simbirtsev A.S.
Russian Journal of Allergy. 2012;9(6):70-71
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POPULATION SPECIFIC CHARACTERISTICS OF GENETIC POLYMORPHISM WITHIN IMMUNE RESPONSE GENES IN RUSSIA
Abramov D.D., Kofiadi I.A., Khaitov M.R., Sergeev I.V., Trofimov D.Y., Gudima G.O., Kadochnikova V.V., Goncharova E.V., Ragimov A.A., Alexeev L.P.
Russian Journal of Allergy. 2012;9(6):72-75
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Interrelation of immune system individual features and hiv infection development after scheduled interruption of antiretroviral theraty in HIV infected pregnant women
Orlova-Morozova E.A., Pronin A.J., Kaminskiy G.D., Lebedeva N.N., Zhukova E.V., Serkov I.L., Gudima G.O., Sidorovich I.G.
Russian Journal of Allergy. 2012;9(6):76-79
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Informational Letter
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Russian Journal of Allergy. 2012;9(6):80-82
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Congresses, Conferences
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Russian Journal of Allergy. 2012;9(6):83-84
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