USE OF MOLECULAR GENETIC METHODS IN THE STUDY OF HEREDITARY PREDISPOSITION TO ATOPIC DISEASES INCHILDREN



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Abstract

Background. Study of the аssociations of susceptibility genes to the development of atopic diseases in children.
Materials and methods. All 325 examined children reside on the territory of the European part of Russia who
by according to surveys, Russian by nationality. Analysis of polymorphism in genes of receptors ADRB2, GRL,
ALOX5, genes of biotransformation - CYP1A1, CYP2C9, CYP2C19, GSTT1, GSTM1, NAT2 as well as the variants of
the genes MTHFR and TNFA was performed in patients suffering from atopic disease and in healthy individuals.
Using Multifactor Dimentionality Reduction method (MDR) it was defined the most significant model of genegene
interaction for the development of atopic disease
Results. Association of the development of atopic diseases with polymorphic variants of the genes: ALOX5 (VNTR)
GRL (1220A > G) TNFA (-308G > A) CYP1A1 (6235T > C) and GSTM1 was identified in surveyed children. The highrisk
alleles and genotypes of developing atopic diseases in pediatric patients were determined. Using Multifactor
Dimentionality Reduction method (MDR) it was defined the most significant model of gene-gene interaction for
the development of atopic disease, including ADRB2 (79 C >G), (46A > G), CYP2C19 (G681A) was defined.
Conclusion. There were identified polymorphic variants of genes and important gene-gene interactions associated
with development of atopic diseases in children.

About the authors

E S Tyumentseva

Email: e_s_t@mail.ru

N V Petrova

I I Balabolkin

V G Pinelis

E S Tumentseva

Children Health Research Centre

Children Health Research Centre

N V Petrova

Research Centre for Medical Genetics, Russian Academy of Medical Sciences

Research Centre for Medical Genetics, Russian Academy of Medical Sciences

I I Balabolkin

Children Health Research Centre

Children Health Research Centre

V G Pinelis

Children Health Research Centre

Children Health Research Centre

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