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Regulat ory T-cells in periphera l blood of pat ientswith br onchial ast hma
- Authors: Kremer EE1, Kirillova NA1, Teplova NV1, Deev IA1, Petrova IV1, Ogorodova LM1, Kremer EE2, Kirillova NA2, Teplova NV2, Deev IA2, Petrova IV2, Ogorodova LM2
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Affiliations:
- Siberian State Medical University
- Issue: Vol 8, No 5 (2011)
- Pages: 21-25
- Section: Articles
- URL: https://rusalljournal.ru/raj/article/view/781
- DOI: https://doi.org/10.36691/RJA781
- ID: 781
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Abstract
T-cells (Treg) in patients with different severity of bronchial asthma (BA).
Methods. We included 19 patients with severe BA, 12 patients with mild BA and 17 healthy donors. The circulating
percentage of CD4+CD25high and CD4+FoxP3+ T-reg in peripheral blood was estimated by the flow cytometry
analysis (FACSCalibur, Becton Dickinson, USA).
Results. Numbers of peripheral blood CD4+CD25high was significantly decreased in mild BA compared to healthy
control. Patients with severe BA had increased levels of CD4+FoxP3+ and CD4+CD25high compared to mild BA.
Conclusion. Our results demonstrate new scientific data on the phenotypic characteristics and the level of T-reg
in peripheral blood at different clinical forms of asthma. This fact confirms the existence of immunoregulatory
control mechanisms of allergic inflammation involving T-reg.
About the authors
E E Kremer
Email: elenakremer@yandex.ru
N A Kirillova
N V Teplova
I A Deev
I V Petrova
L M Ogorodova
E E Kremer
Siberian State Medical UniversitySiberian State Medical University
N A Kirillova
Siberian State Medical UniversitySiberian State Medical University
N V Teplova
Siberian State Medical UniversitySiberian State Medical University
I A Deev
Siberian State Medical UniversitySiberian State Medical University
I V Petrova
Siberian State Medical UniversitySiberian State Medical University
L M Ogorodova
Siberian State Medical UniversitySiberian State Medical University
References
- Buckner J.H. Mechanisms of impaired regulation by CD4(+)CD25(+)FOXP3(+) regulatory T-cells in human autoimmune diseases. Nat. Rev. Immunol. 2010, v. 10 (12), p. 849-859.
- Hori S., Nomura Т., Sakaguchi S. et al. Control of regulatory T-cell development by the transcription factor Foxp3. Science. 2003, v. 299 (5609), p. 1057-1061.
- Jaffar Z., Sivakuru T., Roberts K. CD4+CD25+T-cells regulate airway eosinophilic inflammation by modulating the Th2-cell phenotype. J. Immunol. 2004, v. 172 (6), p. 3842-3849.
- Barron L., Dooms H., Hoyer К.К. et al. Cutting edge: mechanisms of IL-2-dependent maintenance of functional regulatory T-cells. J. Immunol. 2010, v. 185 (11), p. 6426-6430.
- Zheng S.G., Wang J.H., Stohl W. et al. TGF-beta requires CTLA-4 early after T-cell activation to induce FoxP3 and generate adaptive CD4+CD25+ regulatory cells. J. Immunol. 2006, v. 176 (6), p. 3321-3329.
- Provoost S., Maes T., van DurmeY.M.et al. Decreased FoxP3 protein expression in patients with asthma. Allergy. 2009, v. 64, p. 1539-1546.
- Kasprowicz D.J., Smallwood P.S., Tyznik A.J. et al. FoxP3 controls T-dependent immune responses in vivo through regulation of CD4+ T-cell effector function. J. Immunol. 2003, v. 171, p. 1216-1223.
- Turk M. J., Guevara-Patino J.A., Rizzuto G.A. et al. Concomitant tumor immunity to a poorly immunogenic melanoma is prevented by regulatory T-cells. J. Exp. Med., 2004, v. 200 (6), p.771-782.
- McGee H.S., Agrawal D.K. Naturally occurring and inducible T-regulatory cells modulating immune response in allergic asthma. Am. J. Respi. Crit. Care Med. 2009, v. 180 (3), p. 211-225.
- Новиков Д.К., Новикова Т.И. Оценка иммунного статуса. М., Витебск. 1996, 278 с.
- Baecher-Allan C., Brown J.A., Freeman G.J. et al. CD4+CD25 high regulatory cells in human peripheral blood. J. Immunol. 2001, v. 167, p. 1245-1253.
- Zhang Q., Qian F.H., Liu H. et al. Expression of surface markers on peripheral CD4+CD25high T-cells in patients with atopic asthma: role of inhaled corticosteroid. Chin. Med. J. (Engl). 2008, v. 121 (3), p. 205-212.
- Chatila Т.А Role of regulatory T-cells in human diseases. J. Allergy Clin. Immunology. 2005, v. 116, p. 949-959.
- Smyth L.J., Eustace A., Kolsum U. et al. Increased airway T-regulatory cells in asthmatic subjects. Chest. 2010, v. 138 (4), p. 905-912.
- Ling E.M., Smith T., Nguyen X.D. etal. Relation of CD4CD25 regulatory T-cell suppression of allergen-driven T-cell activation to atopic status and expression of allergic disease. Lancet. 2004, v. 363, p. 608-615.
- Lee J.H., Yu H. The levels of CD4+CD25+ regulatory T-cells in pediatric patients with allergic rhinitis and bronchial asthma. Clin, and Exp. Immunology. 2007, v. 148, p. 53-63.
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