Detection of IgE-antibodies to recombinant birch allergens rBet v 1 and rBet v 2 in patients withsensitization to birch pollen



Cite item

Full Text

Open Access Open Access
Restricted Access Access granted
Restricted Access Subscription or Fee Access

Abstract

Background. Allergic diagnosis is based on accurate clinical history and conducting of skin prick tests (sPt)
and specific IgE determination. the past decade recombinant allergen components from pollen of birch are
available for allergen-specific IgE antibody testing. the major allergen of birch tree pollen is Bet v 1; Bet v 2 is a
well described minor allergen.
Methods. 83 patients with birch pollinosis (55 male/28 female, age 4-35 years, mean 15,5) were observed. specific
IgE levels were determined by using the ImmunoCap (Phadia, sweden); skin prick tests with standard aeroallergens
panel was used.
Results. All 83 patients had positive skin tests to birch pollen. the levels of specific IgE to rBet v1 had 87,9% of
patients, sIgE to rBet v 2 (profilin) 14,5% of patients, sIgE to rBet v 1 and rBet v 2 13,3% of patients.
Conclusion. the prevalence of the patients with presence of IgE - antibodies to profiling Bet v2 among citizens
of moscow region, sensitized to birch, accounts 14,5%.

About the authors

M A Mokronosova

Email: mmokronosova@mail.ru

A V Sergeev

E S Korovkina

N G Konyukova

M A Mokronosova

I. Mechnikov Research Institute for Vaccines and Sera, RAMS

I. Mechnikov Research Institute for Vaccines and Sera, RAMS

A V Sergeev

I. Mechnikov Research Institute for Vaccines and Sera, RAMS

I. Mechnikov Research Institute for Vaccines and Sera, RAMS

E S Korovkina

I. Mechnikov Research Institute for Vaccines and Sera, RAMS

I. Mechnikov Research Institute for Vaccines and Sera, RAMS

N G Konjucova

Moscow Research Institute of Pediatric and Child Surgery

Moscow Research Institute of Pediatric and Child Surgery

References

  1. Elfman L., Svensson M., Lidholm J., Pauli G., Valenta R. Different profiles in specific IgE to rBet v 1 and rBet v 2 in patients allergic to birch pollen from six countries. Int. Arch. Allergy Immunol. 1997, v. 113 (1-3), p. 249-251.
  2. Van Ree R., Aalberse R.C. Pollen-vegetable food crossreactivity: serological and clinical relevance of crossreactive IgE. J. Clin. Immunoassay. 1993, v. 16, p. 124-130.
  3. Aalberse R. C., Akkerdaas J. H., van Ree R. Cross-reactivity of IgE antibodies to allergens. Allergy, 2001, v. 56, p. 478-490.
  4. Kondo Y., Urisu A. Oral Allergy Syndrome. Allergol. Int. 2009, v. 4, p. 485-491.
  5. Wensing M., Akkerdaas J.H., van Leeuwen W.A. et al. IgE to Bet v 1 and profilin: crossreactivity patterns and clinical relevance. J. Allergy Clin. Immunol. 2002, v. 110, p. 435-442.
  6. Ebner C., Hirschwehr R., Bauer L. et al. Identification of allergens in fruits and vegetables: IgE cross-reactivities with the important birch pollen allergens Bet v 1 and Bet v 2 (birch profilin). J. Allergy Clin. Immunol. 1995, v. 95, p. 962-969.
  7. Valenta R., Duchene M., Ebner C. et al. Profilins constitute a novel family of functional plant pan-allergens. J. Exp. Med. 1992, v. 175, p. 377-385.
  8. Breiteneder H., Ebner C. Molecular and biochemical classification of plant-derived food allergens. Allergy Clin. Immunol. 2000, v. 106, p. 27-36.
  9. Scheurer S., Wangorsch A., Nerkamp J. et al. Crossreactivity within the profiling panallergen family investigated by comparison of recombinant profilins from pear (Pyr c 4), cherry (Pruav 4) and celery (Api g 4) with birch pollen profiling Bet v 2. J. Chromatogr. B Biomed. Sci. Appl. 2001, v. 756, p. 315-325.
  10. Schmid-Grendelmeier P. Recombinant allergens. For routine use or still only science? Hautarzt. 2010, v. 61 (11), p. 946-953.
  11. http://www.mtallergy.com/sitebuildercontent/sitebuilderfiles/ allergyquestionnaire050107.doc.

Supplementary files

Supplementary Files
Action
1. JATS XML

Copyright © Pharmarus Print Media, 2011



This website uses cookies

You consent to our cookies if you continue to use our website.

About Cookies