The role of mutations in the genes FLG and GSTM1, and cytogenetic aberrations in the developmentof allergic occupational dermatitis in the oilfield workers of the north of Siberia

Background. The objective of the investigation was to determine frequencies of micronucleated cells in the
buccal epithelium in relation to polymorphism of genes FLG and GSTM1 among oilfield workers suffered from
allergic occupational dermatitis.
Methods. There were 209 male oilfield workers examined with cases of allergic professional dermatitis at the age
of 21-39 years old. as a control group there were used 207 male workers without allergic occupational dermatitis.
we used genealogical method, micronucleous test and polymerase chain reaction to detect restriction fragments
of genes GSTM1 and FLG.
Results. it was detected that oilfield workers with genotypes contented homozygous mutated alleles GSTM1 (0/0)
gene showed the higher frequency of micronucleated epithelial cells. it was not detected for FLG gene allele
variants. among the examined oilfield workers with allergic occupational dermatitis there was nobody that would
have both mutated alleles of FLG (282de14/282de14) and homozygous GSTM1 (0/0) genes, while in the control
group we found some individuals of this type.
Conclusions. The significantly higher levels of cytogenetic aberrations in the oilfield workers suffered from allergic
occupational dermatitis are the result of combined effects of various factors on the genome. The investigation
can provide the basis for the elaboration of new scientific criteria for hiring staff in oilfields of west siberia.

GSTM1, allergic occupational dermatitis, FLG, GSTM1, gene polymorphism, oilfield of siberia, mutations, micronucleus test, natural factors