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THE INFLUENCE OF TOPICAL GLUCOCORTICOSTEROIDS ONCYTOKINE GENE EXPRESSION IN THE SKIN AND PERIPHERALBLOOD OF ATOPIC DERMATITIS PATIENTS
- Authors: Filimonova T.M.1, Elisyutina OG1, Fedenko ES1, Burmenskaya OV1, Boldyreva MN1, Filimonova TM2, Elisyutina OG2, Fedenko ES2, Burmenskaya OV3, Boldyreva MN2
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Affiliations:
- Institute of Immunology
- Scientific Center for Obstetrics, gynecology and perinatology named after V.I. kulakov
- Issue: Vol 8, No 3 (2011)
- Pages: 19-30
- Section: Articles
- Submitted: 10.03.2020
- Published: 15.09.2011
- URL: https://rusalljournal.ru/raj/article/view/779
- DOI: https://doi.org/10.36691/RJA779
- ID: 779
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Abstract
skin and peripheral blood of atopic dermatitis (AD) patients comparing with control.
Material and methods. 40 AD patients were included in the study and divided into 2 groups. group 1 patients
were given continuous course of mometasone furoate 0,1% cream treatment for 14 days. group 2 patients were
given indifferent emollient elobase cream for 14 days. Clinical efficacy of the treatment was assessed on the
following features: SCORAD index, Investigators global Assessment (IgA) score and subjective assessment of
itch and dryness of the skin according to skin area to be studied. Skin samples and peripheral blood of atopic
dermatitis were used as material for immunological study. Interleukin (IL)1B, IL2, IL2r, IL4, IL5, IL6, IL7, IL8, IL10,
IL 12A, IL12B, IL15 (total), IL15 , IL17A, IL18, IL23, IL28, IL29, Interferon (IFN)-, Tumor necrosis factor (TNF),
Transforming growth factor beta 1 (TGFB1), forkhead box P3 (FOXP3) gene expression were defined in the skin
and peripheral blood of AD patients by realtime reverse transcription polymerase chain reaction (RT-pCR).
Results. positive clinical effect was found with all AD patients on the mometasone furoate 0,1% cream therapy
background for 14 days and also dryness, rushes and skin itch decreased. Stаtistical significant decrease of proinflammatory
cytokines IL2, IL2r, IL5, IL8, IL12В, IL23, IFN- gene expression was marked. They are the markers
of chronic inflammation and Th1 immune response. Studying peripheral blood after mometasone furoate 0,1%
cream treatment increase of TGFB1, FOXP3 gene expression level was found. no significant changes of cytokine
gene expression in AD patients, who got elobase cream were found.
Conclusion. Antiinflammatory activity of mometasone furoate 0,1% cream was shown by its influence on proinflammatory
cytokines IL2, IL2r, IL5, IL8, IL12В, IL23, IFN- gene expression in the skin and mechanisms of
immune response in moderate and severe AD patients.
About the authors
Tat'yana Mikhaylovna Filimonova
Email: tatfil-doc@yandex.ru
O G Elisyutina
E S Fedenko
O V Burmenskaya
M N Boldyreva
T M Filimonova
Institute of ImmunologyInstitute of Immunology
O G Elisyutina
Institute of ImmunologyInstitute of Immunology
E S Fedenko
Institute of ImmunologyInstitute of Immunology
O V Burmenskaya
Scientific Center for Obstetrics, gynecology and perinatology named after V.I. kulakovScientific Center for Obstetrics, gynecology and perinatology named after V.I. kulakov
M N Boldyreva
Institute of ImmunologyInstitute of Immunology
References
- Hogan A.D., Burks A.W. Epidermal Langerhans cells and their function in the skin immune system. Ann. Allergy Asthma Immunol. 1995, v. 71 (1), p. 5-10.
- Hauser C. The interaction between Langerhans cells and СD4+ T-cell. J. Dermatol. 1992, v. 19 (11), p. 722-725.
- Белова О.В., Арион В.Я. Иммунологическая функция кожи и нейроиммунокожная система. Аллергология и иммунология. 2006, т. 7, № 4, с. 492-497.
- Bieber Thomas. Mechanisms of Disease Atopic Dermatitis. N. Engl. J. Med. 2008, v.358, p.1483-1494.
- Agace W.W., Higgins J.M., Sadasivan B. et al. T-lymphocyte - epithelial cell interactions: integrin aEb7, LEEP-CAM and chemokines. Curr. Opin. Cell Biol. 2000, v. 12, р. 563-568.
- Ярилин А.А. Взаимодействие эпителиальных и лимфоидных клеток барьерных тканей в норме и при патологии. В сб.: Современные проблемы аллергологии, иммунологоии и иммунофармакологии. М., 2001 (1), с. 261-289.
- Elkord E. Role of regulatory T-cells in allergy: implications for therapeutic strategy. Inflamm. Allergy. Drug Targets. 2006, v. 5 (4), p. 211-212.
- Cooper K.D. Atopic Dermatitis: recent trends in pathogenesis and therapy. J. Invest Dermatol. 2003, v. 102, p. 128-137.
- Candi E., Schmidt R., Melino G. The cornified envelope: a model of cell death in the skin. Nat. Rev. Mol. Cell Biol. 2006, v. 6 (4), р. 328-340.
- Komine M. Analysis of the mechanism for the development of allergic skin inflammation and the application for its treatment: keratinocytes in atopic dermatitis their pathogenicinvolvement. J. Pharmacol. Sci. 2009, v. 110 (3), p. 260-264
- Nelson H.S. Advances in upper airway diseases and allergen immunotherapy. J. Allergy Clin. Immunol. 2004, v. 113, p. 635-642.
- Mudde G.C., Van Reijsen F.C., Boland G.J. et al. Allergen presentation by epidermal Langerhans cells from patients with atopic dermatitis is mediated by IgE. Immunol. 1990, v. 69, p. 41-335.
- Romani N., Gruner S., Brang D. et al. Proliferating dendritic cells progenitors in human blood. J. of experim. medicine. 2004, v. 180, p. 83-93.
- Leung Y.M., Soter N.A. Cellular and immunologic mechanisms in atopic dermatitis. J. Am. Acad. Dermatol. 2001, v. 44, p. 1-12.
- Akdis C.A. Review article Diagnosis and treatment of atopic dermatitis in children and adults: European Academy of Allergology and Clinical Immunology. American Academy of Allergy, Asthma and Immunology. PRACTALL Consensus report. Allergy. 2006, v. 61, p. 969-987.
- Бартон Б., Яаквей Д., Смит С., Зигель М. Ингибирование цитокинового синтеза новым стероидом мометазона фуроатом. Вестн. дерматол. и венерол. 1999, № 3, с. 43-45.
- Гамаюнов Б.Н., Короткий Н.Г., Тихомиров А.А., Сучкова Т.Н. Мометазона фуроат и гидрокортизона 17-бутират в наружной терапии атопического дерматита у детей. РМЖ. 2008, т. 16, № 18, с. 1183.
- Berardesca E., Barbareschi M., Veraldi S. еt al. Evaluation of efficacy of skin lipid mixture in patients with irritant contact dermatitis, allergic contact dermatitis, or atopic dermatitis: a multicenter study. Contact Dermatitis. 2001, v. 45 (5), p. 280-285.
- Simpson E.L., Berry T.M., Brown P.A., Hanifin J.M. A pilot study of emollient therapy for the primary prevention of atopic dermatitis. J. Am. Acad. Dermatol. 2010, v. 63 (4), p. 587-593.
- Mohammed D., Matts P.J., Hadgraft J., Lane M.E. Influence of Aqueous Cream on corneocyte size, maturity, skin protease activity, protein content and Trans-Epidermal Water Loss. Br. J. Dermatol. 2011.
- Hanifin J.M., Rajka G. Diagnostic features of atopic dermatitis. Acta Derm. Venerol. 1980, v. 92, p. 44-47.
- Ребриков Д.В., Саматов Г.А., Трофимов Д.Ю., Семёнов П.А и соавт. ПЦР в «реальном времени». М., «БИНОМ». Лаборатория знаний. 2009, c. 115.
- Europian Task Force on Atopic Dermatitis. Severity scoring of atopic dermatitis: the SCORAD index. Dermatology. 1993, v. 186, p. 23-31.
- Ito Y., Adachi Y., Makino T. et al. Expansion of FOXP3-positive CD4+CD25+ T-cells associated with disease activity in atopic dermatitis. Ann. Allergy Asthma Immunol. 2009, v. 103 (2), p. 160-165.
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