Polymorphism of the cytokine genes (H4, ИМ) and peculiarities of immune response depending on the control over atopic bronchial asthma in children

  • Authors: Smolnikova MV1, Smirnova SV1, Tyutina OS1, Bychkovskaya SV2
  • Affiliations:
    1. Federal State Budget Institution «Scientific Research Institute for Medical Problems of the North» of Siberian Division of Russian Academy of Medical Sciences
    2. State budget institution of higher professional education «Krasnoyarsk State Medical University named after professor V.F. Voino-Yasenetzkiy» Ministry of Health of the Russian Federation
  • Issue: Vol 10, No 2 (2013)
  • Pages: 23-28
  • Section: Articles
  • URL: https://rusalljournal.ru/raj/article/view/608
  • DOI: https://doi.org/10.36691/RJA608
  • Cite item
Open Access Open Access
Restricted Access Access granted
Restricted Access Subscription or Fee Access

Abstract


Background. The aim of this study was to investigate the main indices of humoral and cellular immunity, the levels of cytokines (IL-2, IL-4, IL-10, TNFa) and polymorphism of promoter regions of cytokine genes (IL4 and IL10) in children with atopic bronchial asthma (ABA) with different levels of disease control. Methods. We have analyzed 110 children with ABA - controlled (CABA, n=59) and uncontrolled (UABA, n=51) and healthy children (control group, n=52). Parameters of cellular immunity were determined by fluorescence microscopy using monoclonal antibodies to surface receptors. Parameters of humoral immunity and cytokine levels in the samples of serum were measured by ELISA. Genotyping of single-nucleotide polymorphisms in the IL4 (С-590Т) and IL10 (С597А) genes was performed by PCR. Results. The level of TNFa and the relative amount of CD8+ cells was increased, while the counts of CD3+ cells and the relative amount of CD4+ cells was decreased in UABA as compared to CABA. In CABA, lower concentration of the IL-10 in serum associated with the IL10 A-597 allele was observed. The IL4 T-590 allele tends to be associated with non-controlled ABA. Conclusion. The level of TNFa, and the CD3+, CD4+ and CD8+ cell counts were identified as markers of uncontrolled course of ABA in children. Polymorphic variants of IL4 and IL10 genes can be considered as candidate markers of uncontrolled ABA.

Restricted Access

M V Smolnikova

Federal State Budget Institution «Scientific Research Institute for Medical Problems of the North» of Siberian Division of Russian Academy of Medical Sciences

Email: smarinv@ya.ru
Krasnoyarsk

S V Smirnova

Federal State Budget Institution «Scientific Research Institute for Medical Problems of the North» of Siberian Division of Russian Academy of Medical Sciences

Krasnoyarsk

O S Tyutina

Federal State Budget Institution «Scientific Research Institute for Medical Problems of the North» of Siberian Division of Russian Academy of Medical Sciences

Krasnoyarsk

S V Bychkovskaya

State budget institution of higher professional education «Krasnoyarsk State Medical University named after professor V.F. Voino-Yasenetzkiy» Ministry of Health of the Russian Federation

Krasnoyarsk

  1. Глобальная стратегия лечения и профилактики бронхиальной астмы (пересмотр 2007 г.). Под ред. А.Г. Чучалина. М., «Атмосфера». 2008, с. 108.
  2. Баранов А.А., Хаитов Р.М. Аллергология и иммунология. М., «Союз педиатров России». 2010, с. 248.
  3. Козлов В.А., Борисов А.Г., Смирнова С.В., Савченко А.А. Практические аспекты диагностики и лечения иммунных нарушений. Руководство для врачей. Новосибирск, «Наука». 2009, с. 274.
  4. Кетлинский С.А., Симбирцев А.С. Цитокины. СПб., «Фолиант». 2008, с. 552.
  5. Смирнова С.В., Зенкина Л.В., Игнатова И.А., Кадричева С.Г. Концентрация IL-2, IL-4, IL-6 и IFN-γ в периферической крови и назальных смывах при респираторной атопии и псевдоатопии. Рос. Аллерголог. Журн. 2005, № 1, с. 30-34.
  6. Bidwell J., Keen L., Gallageher G. et al. Cytokine gene polymorphism in human disease: on-line databases. Genes and Immunity. 1999, v. 1, p. 3-19.
  7. Kamali-Sarvestani E., Ghayomi M.A., Nekoee A. Association of TNFa- 308 G/A and IL-4 -589 C/T gene promoter polymorphisms with asthma susceptibity in the south of Iran. J. Investig Allergol. Clin. Immunol. 2007, v. 17 (6), p. 361-366.
  8. Emonts M. Genetic polymorphisms in immunoresponse genes TNFa IL-6, IL-10, and TLR4 are associated with recurrent acute otitis media. Pediatrics. 2007, v. 120 (4), p. 814-823.
  9. Li Y., Guo B., Zhang L. et al. Association between C-589T polymorphisms of interleukin-4 gene promoter and asthma: a meta-analysis. Respir. Med. 2008, v. 102 (7), p. 984-992.
  10. Kim K.W., Lee K.E., Hong J.Y. et al. Involvement of IL-10 gene promoter polymorphisms in the susceptibity for childhood asthma. Lung. 2011, v. 189 (5), p. 417-423.
  11. Коненков В.И., Смольникова М.В. Структурные основы и функциональная значимость аллельного полиморфизма генов цитокинов человека и их рецепторов. Мед. иммунол. 2003, № 1-2, c. 11-28.
  12. Симбирцев А.С., Громова А.Ю. Функциональный полиморфизм генов регулярных молекул воспаления.Цитокины и воспаление. 2005, № 1, c. 3-10.
  13. Karjalainen J., Hulkkonen J., Nieminen M.M. et al. Interleukin-10 gene promoter region polymorphism is associated with eosinophil count and circulating immunoglobulin E in adult asthma. Clin. Exp. Allergy. 2003, v. 33 (1), p. 78-83.
  14. Тютина О.С., Смирнова C.B., Смольникова М.В., Ильенкова Н.А. Клинико-иммунологические особенности атопической бронхиальной астмы в зависимости от уровня контроля над заболеванием у детей. Бюллетень Восточно-Сибирского научного центра СО РАМН. 2012, № 3, c. 204-207.
  15. Смольникова М.В., Прокофьев В.Ф., Сизякина Л.П. и соавт. Аллельные варианты генов IL-4, IL-10 и TNFα при ВИЧ-инфекции. Цитокины и воспаление. 2002, № 1, с. 35-39.

Views

Abstract - 7

PDF (Russian) - 0

PlumX

Dimensions

Refbacks

  • There are currently no refbacks.

Copyright (c) 2013 Russian Allergological Journal

Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

This website uses cookies

You consent to our cookies if you continue to use our website.

About Cookies