ACE and PLA2G7genes expression in children with a history of food anaphylaxis



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Abstract

Background. The aim of this study was to evaluate transcriptional activity of PLA2G7 (platelet-activating factor acetylhydrolase) and ACE (аngiotensin-сonverting enzyme) genes in patients with a history of anaphylaxis. Methods. 97 children were enrolled in the study: 27 children with a history of food anaphylaxis; 35 children with atopic dermatitis; 35 children without allergic diseases: 26 children were IgE-negative, 9 children were IgE-positive («Phadia 100»; «Phadia AB», Sweden). RNA was extracted from blood samples with Trizol RNA Prep kits («Isogen», Russia). cDNA production was performed with MMLV RT kit («Evrogen», Russia). Real-time PCR was held with CFX-96 device («Bio-Rad», USA) and qPCRmix-HS PCR mix («Evrogen», Russia). Oligonucleotide primers and probes were synthesized at «DNK-Sintez» (Russia). Results. In children with a history of food anaphylaxis decrease in PLA2G7 gene expression was detected comparing to control groups (p<0,05). Transcriptional activity of PLA2G7 and detection rate of ACE gene expression were lower in children with moderate/severe anaphylaxis comparing with mild anaphylaxis children (p<0,05). The study revealed relationship of PLA2G7 and ACE gene expression and clinical features of anaphylaxis. Conclusion. Reduction of blood cells PLA2G7 and ACE mRNA levels in children with a history of food anaphylaxis may be considered to be a biomarker of severe anaphylaxis.

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About the authors

N V Esakova

Pirogov Russian National Research Medical University

Email: env007@rambler.ru
Research and Clinical Institute for Pediatrics Moscow

T S Okuneva

Pirogov Russian National Research Medical University

Research and Clinical Institute for Pediatrics Moscow

E K Saakyan

Lomonosov Moscow State University

Department of Genetics, Faculty of Biology Moscow

N S Kondratieva

Lomonosov Moscow State University

Department of Genetics, Faculty of Biology Moscow

Z G Kokaeva

Lomonosov Moscow State University

Department of Genetics, Faculty of Biology Moscow

E A Klimov

Lomonosov Moscow State University; Universitydiagnostic Laboratory

Department of Genetics, Faculty of Biology Moscow

A N Pampura

Pirogov Russian National Research Medical University

Research and Clinical Institute for Pediatrics Moscow

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