ACE and PLA2G7genes expression in children with a history of food anaphylaxis

Full Text

Open Access Open Access
Restricted Access Access granted
Restricted Access Subscription or Fee Access

Abstract

Background. The aim of this study was to evaluate transcriptional activity of PLA2G7 (platelet-activating factor acetylhydrolase) and ACE (аngiotensin-сonverting enzyme) genes in patients with a history of anaphylaxis. Methods. 97 children were enrolled in the study: 27 children with a history of food anaphylaxis; 35 children with atopic dermatitis; 35 children without allergic diseases: 26 children were IgE-negative, 9 children were IgE-positive («Phadia 100»; «Phadia AB», Sweden). RNA was extracted from blood samples with Trizol RNA Prep kits («Isogen», Russia). cDNA production was performed with MMLV RT kit («Evrogen», Russia). Real-time PCR was held with CFX-96 device («Bio-Rad», USA) and qPCRmix-HS PCR mix («Evrogen», Russia). Oligonucleotide primers and probes were synthesized at «DNK-Sintez» (Russia). Results. In children with a history of food anaphylaxis decrease in PLA2G7 gene expression was detected comparing to control groups (p<0,05). Transcriptional activity of PLA2G7 and detection rate of ACE gene expression were lower in children with moderate/severe anaphylaxis comparing with mild anaphylaxis children (p<0,05). The study revealed relationship of PLA2G7 and ACE gene expression and clinical features of anaphylaxis. Conclusion. Reduction of blood cells PLA2G7 and ACE mRNA levels in children with a history of food anaphylaxis may be considered to be a biomarker of severe anaphylaxis.

Full Text

Restricted Access

About the authors

N V Esakova

Pirogov Russian National Research Medical University

Email: env007@rambler.ru
Research and Clinical Institute for Pediatrics Moscow

T S Okuneva

Pirogov Russian National Research Medical University

Research and Clinical Institute for Pediatrics Moscow

E K Saakyan

Lomonosov Moscow State University

Department of Genetics, Faculty of Biology Moscow

N S Kondratieva

Lomonosov Moscow State University

Department of Genetics, Faculty of Biology Moscow

Z G Kokaeva

Lomonosov Moscow State University

Department of Genetics, Faculty of Biology Moscow

E A Klimov

Lomonosov Moscow State University; Universitydiagnostic Laboratory

Department of Genetics, Faculty of Biology Moscow

A N Pampura

Pirogov Russian National Research Medical University

Research and Clinical Institute for Pediatrics Moscow

References

  1. Kounis N.G., Soufras G.D., Hahalis G. Anaphylactic Shock: Hypersensitivity Associated Syndrome Seems to be the Primary Oause. N. Am. J. Med. Sci. 2013, v. 5, p. 631-636.
  2. Vadas P., Perelman B., Liss G. Platelet-activating factor, histamine, and tryptase levels in human anaphylaxis. J. Allergy Сііп. Immunol. 2013, v. 131, p. 144-149.
  3. Pravettoni V., Piantanida M., Primavesi L. et al. Basal platelet-activating factor acetylhydrolase: Prognostic marker of severe Hymenoptera venom anaphylaxis. J. Allergy Clin. Immunol. 2014, v. 133, p. 1218-1220.
  4. Summers G.W., Pumphrey R.S., Woods C.N. et al. Factors predicting anaphylaxis to peanuts and tree nuts in patients referred to a specialist center. J. Allergy Сііп. Immunol. 2008, v. 121, p. 632-638.
  5. Varney V.A., Warner A., Ghosh A. et al. IgE-mediated anaphylaxis to foods, venom, and drugs: influence of serum angiotensin converting enzyme levels and genotype. J. Allergy (Cairo). 2012, v. 4, p. 1-9.
  6. Muraro G.R., Clark A., Eigenmann P. et al. The management of anaphylaxis in childhood: position paper of the European academy of allergology and clinical immunology. Allergy. 2007, v. 62, p. 857-871.
  7. Vadas P., Gold M., Perelman B. et al. Platelet-activating factor, PAF acetylhydrolase, and severe anaphylaxis. N. Engl. J. Med. 2008, v. 358, p. 28-35.
  8. Vadas P., Gold M., Liss G. et al. PAF acetylhydrolase deficiency predisposes to fatal anaphylaxis. J. Allergy Сііп. Immunol. 2003, v. 111, р. 206.
  9. Arias K., Baig M., Golangelo M. et al. Concurrent blockade of platelet-activating factor and histamine prevents life-threatening peanut-induced anaphylactic reactions. J. Allergy Clin. Immunol. 2009, v. 124, p. 307-314.
  10. Fukuda Y., Kawashima H., Saito K. et al. Effect of human plasma-type platelet-activating factor acetylhydrolase in two anaphylactic shock models. Eur. J. Pharmacol. 2000, v. 390, p. 203-207.
  11. Simons F.E., Ardusso L.R., Bilò M.B. et al. World Allergy Organization anaphylaxis guidelines: summary. J. Allergy Clin. Immunol. 2011, v. 127, p. 587-593.
  12. Hiramoto M., Yoshida H., Imaizumi T. et al. A mutation in plasma platelet-activating factor acetylhydrolase (Val279->Phe) is a genetic risk factor for stroke. Stroke. 1998, v. 28, p. 2417-2420.
  13. Satoh N., Asano K., Naoki K.et al. Plasma platelet-activating factor acetylhydrolase deficiency in Japanese patients with asthma. Am. J. Respir. Crit. Care Med. 1999, v. 159, p. 974-979.
  14. Kruse S., Mao X.Q., Heinzmann A. et al. The Ile198Thr and Ala379Val variants of plasmatic PAF-ncetylhydrolase impair catalytical activities and are associated with atopy and asthma. Am. J. Hum. Genet. 2000, v. 66, p. 1522-1530.
  15. Braquet P., Touqui L., Shen T.Y., Vargaftig B.B. Perspectives in platelet-activating factor research. Pharmacol. Rev. 1987, v. 39, p. 97-145.
  16. Yamada Y., Ichihara S., Izawa H. et al. Association of a G994->T (Val279->Phe) polymorphism of the plasma platelet-activating factor acetylhydrolase gene with myocardial damage in Japanese patients with nonfamilial hypertrophic cardiomyopathy. J. Hum. Genet. 2001, v. 46, p. 436-441.
  17. Choi I.H., Ha T.Y., Lee D.G. et al. Occurrence of disseminated intravascular coagulation (DIC) in active systemic anaphylaxis: role of platelet-activating factor. Clin. Exp. Immunol. 1995, v. 100, p. 390-394.
  18. Hermann K., Ring J. The renin-angiotensin system and hymenoptera venom anaphylaxis. Clin. Exp. Allergy. 1993, v. 23, p. 762-769.
  19. Hermann K., Tschirschnitz M., Eschenbach C., Ring J. Histamine, tryptase, norepinephrine, angiotensinogen, angiotensin-converting enzyme, angiotensin I and II in plasma of patients with hymenoptera venom anaphylaxis. Lnt. Arch. Allergy Immunol. 1994, v. 104, p. 379-384.

Statistics

Views

Abstract: 118

Article Metrics

Metrics Loading ...

Dimensions

PlumX


Copyright © Pharmarus Print Media, 2015



This website uses cookies

You consent to our cookies if you continue to use our website.

About Cookies