THE DIAGNOSTIC SIGNIFICANCE OF IMMUNOLOGICAL PARAMETERS OF THE PATIENTS WITH CHRONIC RHINOSINUSITIS WITH NASAL POLYPS



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Abstract

Background. Investigation of changes in immunological parameters is required to detect the degree of adequate immune system reactions to pathology process and to determine the answer to provided treatment. Taking into account high immunogramm cost differentiated approach is necessary in each specific case basing on economic and diagnostic effectiveness. Now chronic rhinosinusitis with nasal polyps (CRSwNP) is still poorly investigated. In the same clinical situation it is difficult to predict the pathology process development and length of remission period after surgical or conservative treatment. The most substantial difficulties in determining tactics of patients’ management in CRSwNP are associated with asthma, atopy or intolerance to nonsteroidal antiinflammatory drugs (NSAIDs). To determine the diagnostic significance of indicators of systemic cellular immunity for pathology process development forecasting in CRSwNP patients. Methods. CD3, CD4, CD8, CD16, CD19, CD25, CD27, CD45, CD45R0, CD45RA, CD56 and CD127 subpopulations of lymphocytes were examined in peripheral blood of 20 patients (age 46,7±16,06) with bilateral CRSwNP in remission using a flow cytometry method. Results. The study revealed rise in absolute or relative quantity of activated T-lymphocytes (CD3+CD25+), NK cell number (CD3 CD16+CD56+) and T-regulatory (T-reg) cells (CD4+CD25brightCD127lowtoneg), rise in absolute count of memory B-cells (CD19+CD5-CD27+) and NKT-cells (CD16+CD56+CD3+) in CRSwNP patients. An effort to make forecast for the development of CRSwNP due to change in NK (CD3-CD16+CD56+), activated NK (CD3-CD8+) and T-reg (CD4+CD25brightCD127lowtoneg) failed. Conclusion. Study of the cellular immune response to determine the tactics of patients’ management in CRSwNP and predict pathology process development is not a sufficiently informative method.

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About the authors

E L Savlevich

Central State Medical Academy of Department for Presidential Affairs of the Russian Federation

Email: savllena@gmail.com
21, Marshala Timoshenko str., Moscow, 121359, Russia

O M Kurbacheva

NRC Institute of Immunology FMBA of Russia

24, Kashirskoe shosse, Moscow,115478, Russia

S V Khaidukov

M.M. Shemyakin and Yu.A. Ovchinnikov Institute of Bioorganic Chemistry of the Russian Academy of Sciences

16/10, Miklukho-Maklaya str., Moscow, 117997, Russia

A N Gerasimov

First Moscow State Medical University (Sechenov University)

2/4, Bolshaya Pirogovskaya str., Moscow, 119991, Russia

M E Amanturlieva

NRC Institute of Immunology FMBA of Russia

24, Kashirskoe shosse, Moscow,115478, Russia

A S Simbirtcev

State Research Institute of Highly Pure Biopreparations FMBA of Russia

7, Pudozhskaya str., St.-Petersburg, 197110, Russia

References

  1. Воробьев АА, Быков АС, Караулов АВ. Иммунология и аллергология: цветной атлас: учеб. Пособие для мед. вузов. М.: Практическая медицина; 2006.
  2. Ахматова НК, Киселевский МВ. Врожденный иммунитет противоопухолевый и противоинфекционный. М.: Практическая медицина, 2008.
  3. Москалев АВ, Сбойчиков ВБ. Инфекционная иммунология. СПб.: Фолиант, 2006.
  4. Хайдуков СВ, Зурочка АВ, Тотолян AA, Черешнев ВА. Основные и малые популяции лимфоцитов периферической крови человека и их нормативные значения (методом многоцветного цитометрического анализа). Медицинская иммунология, 2009;11:227-238.
  5. Savelkoul H. Immune parameters in high-risk atopic individuals during early childhood. Am. J. Respir. Crit. Care Med. 2000;162(3):100-104. doi: 10.1164/ajrccm.162.supplement_2.ras-9.
  6. Castro C, Gourley M. Diagnostic testing and interpretation of tests for autoimmunity. J. Allergy Clin. Immunol. 2010;125:238-247. doi: 10.1016/j.jaci.2009.09.041.
  7. Земсков МА, Хорошилов АА, Ильина ЕМ, Домнич ОА. Особенности изменения иммунного статуса при гнойно-воспалительных заболеваниях. Вестник экспериментальной клинической хирургии. 2011;24(3): 468-472.
  8. Chew T, Taylor KE, Mossman KL. Innate and adaptive immune responses to herpes simplex virus. Viruses. 2009;1:979-1002. doi: 10.3390/v1030979.
  9. Новикова ИА, Злотникова МВ. Субпопуляционный состав лимфоцитов у больных герпетической инфекцией тяжелого течения. Медицинская иммунология. 2010;(45):331336.
  10. Савлевич ЕЛ, Бродовская ОБ, Ремизова ИИ, Чистякова ГН, Ищенко АМ, Симбирцев АС. Клиникоиммунологическая эффективность применения новой аэрозольной формы рекомбинантного интерферона-a2b в лечении больных с острыми назофарингитами. Цитокины и воспаление. 2010;(9):4956.
  11. Герасимов АН, Морозова НИ. Параметрические и непараметрические методы в медицинской статистике. Эпидемиология и вакцинопрофилактика, 2015;(84):612.
  12. Sharma S, Watanabe S, Sivam A, Wang J, Neuwirth SJ, Perez RI, De Tineo M, Baroody FM, Naclerio RM, Pinto JM. Peripheral blood and tissue T-regulatory cells in chronic rhinosinusitis. Am. J. Rhinol. Allergy. 2012;26:371-379. DOI: 10.2500/ ajra.2012.26.3800.
  13. Miljkovic D, Psaltis A, Wormald PJ, Vreugde S. T-regulatory and Th17-cells in chronic rhinosinusitis with polyps. Int. Forum Allergy Rhinol. 2016;6:826-834. doi: 10.1002/alr.21742.
  14. Pant H, Hughes A, Schembri M, Miljkovic D, Krumbiegel D. CD4+ and CD8+ regulatory T-cells in chronic rhinosinusitis mucosa. Am. J. Rhinol. Allergy. 2014;28:83-89. doi: 10.2500/ajra.2013.28.4014.
  15. Kim YM, Munoz A, Hwang PH, Nadeau KC. Migration of regulatory T-cells toward airway epithelial cells is impaired in chronic rhinosinusitis with nasal polyposis. Clin. Immunol. 2010;137:111-121. doi: 10.1016/j.clim.2010.05.013.
  16. Van Bruaene N, Perez-Novo CA, Basinski TM, Van Zele T, Holtappels G, De Ruyck N, Schmidt-Weber C, Akdis C, Van Cauwenberge P, Bachert C, Gevaert P. T-cell regulation in chronic paranasal sinus disease. J. Allergy Clin. Immunol. 2008;121:1435-1441. DOI: 10.1016/j. jaci.2008.02.018.
  17. Kim JH, Kim GE, Cho GS, Kwon HJ, Joo CH, Kim HS, Jang YJ. Natural killer cells have impaired effector functions. PLoS One. 2013;8:e77177. doi: 10.1371/journal.pone.0077177.
  18. Kim JH, Choi GE, Lee BJ, Kwon SW, Lee SH, Kim HS, Jang YJ. Natural killer cells regulate eosinophilic inflammation in chronic rhinosinusitis. Sci Rep. 2016;8;6:27615. doi: 10.1038/srep27615.

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