Cationic peptides as promising compounds for the treatment of bacterial complications in atopic dermatitis: Antibacterial activity assessment

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Abstract

BACKGROUND: The decrease in the effectiveness of antibiotics against the background of resistance of microorganisms aggravates the therapy of atopic dermatitis complicated by bacterial infection and actualizes the development of new antimicrobial agents.

AIM: To develop, synthesize, and evaluate the antibacterial activity of cationic peptides and an aqueous solution of fullerene C60 to create drugs based on them that will have a spectrum of biological activity, including anti-inflammatory, antiallergic, and antibacterial activities.

MATERIALS AND METHODS: This study analyzed the developed linear and dendrimer cationic peptides, whose structure was confirmed by matrix-assisted laser desorption ionization–time of flight mass spectrometry. An aqueous solution of fullerene C60 was obtained using a uniquely developed and patented technology. Antibacterial activity was assessed by diffusion into agar using disks (screening) and serial dilution, which was used to determine the minimum bactericidal concentration of the studied compounds.

RESULTS: Moreover, 42 cationic peptides with various structures were developed and synthesized. The molecular weight of the peptides did not exceed 5,000 Da. They contained 7–25 amino acids with charges from +5 to +16. Screening was carried out through diffusion into agar using disks and revealed 15 peptides that showed activity against Escherichia coli Dh5a. Thus, using the method of counting colonies, the peptides AB-14, AB-17, and AB-18 showed bactericidal activity relative to E. coli Dh5a in concentrations of 0.03, 0.15, and 0.74 mM, respectively, which exceeded that of ampicillin (0.74 mM) several times. Analysis of an aqueous solution of fullerene C60 did not reveal its antibacterial activity.

CONCLUSIONS: The antibacterial activity of the resulting peptides makes them promising for the development of antibacterial therapeutic agents.

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About the authors

Anastasia A. Galkina

National Research Center ― Institute of Immunology Federal Medical-Biological Agency of Russia

Email: anastasia.a.galkina@gmail.com
ORCID iD: 0000-0003-4521-0813
SPIN-code: 7329-0197
Russian Federation, Moscow

Darya K. Bolyakina

National Research Center ― Institute of Immunology Federal Medical-Biological Agency of Russia

Email: bolyakina.dasha@gmail.com
ORCID iD: 0009-0006-2223-1514
Russian Federation, Moscow

Anastasia V. Shatilova

National Research Center ― Institute of Immunology Federal Medical-Biological Agency of Russia

Email: av.timofeeva@nrcii.ru
ORCID iD: 0000-0003-3780-2878
SPIN-code: 1988-1536
Russian Federation, Moscow

Artem A. Shatilov

National Research Center ― Institute of Immunology Federal Medical-Biological Agency of Russia

Email: aa.shatilov@nrcii.ru
ORCID iD: 0000-0002-4675-8074
SPIN-code: 6768-5796
Russian Federation, Moscow

Marina O. Babikhina

National Research Center ― Institute of Immunology Federal Medical-Biological Agency of Russia

Email: mbabihina@gmail.com
ORCID iD: 0009-0000-5935-1647
SPIN-code: 4621-0268
Russian Federation, Moscow

Alla K. Golomidova

Federal Research Centre "Fundamentals of Biotechnology" of the Russian Academy of Sciences

Email: alla_golomidova@mail.ru
ORCID iD: 0000-0001-9893-0270
SPIN-code: 9954-3759

Cand. Sci. (Biol.)

Russian Federation, Moscow

Alexandra A. Nikonova

National Research Center ― Institute of Immunology Federal Medical-Biological Agency of Russia

Email: aa.nikonova@nrcii.ru
ORCID iD: 0000-0001-9610-0935
SPIN-code: 1950-5594

Cand. Sci. (Biol.)

Russian Federation, Moscow

Sergey M. Andreev

National Research Center ― Institute of Immunology Federal Medical-Biological Agency of Russia

Email: andsergej@yandex.ru
ORCID iD: 0000-0001-8297-579X
SPIN-code: 2542-5260

Cand. Sci. (Chem.)

Russian Federation, Moscow

Dmitry A. Kudlay

National Research Center ― Institute of Immunology Federal Medical-Biological Agency of Russia

Email: D624254@gmail.com
ORCID iD: 0000-0003-1878-4467
SPIN-code: 4129-7880

MD, Dr. Sci. (Med.)

Russian Federation, Moscow

Nadezda N. Shershakova

National Research Center ― Institute of Immunology Federal Medical-Biological Agency of Russia

Email: nn.shershakova@nrcii.ru
ORCID iD: 0000-0001-6444-6499
SPIN-code: 7555-5925

Cand. Sci. (Biol.)

Russian Federation, Moscow

Musa R. Khaitov

National Research Center ― Institute of Immunology Federal Medical-Biological Agency of Russia; The Russian National Research Medical University named after N.I. Pirogov

Author for correspondence.
Email: mr.khaitov@nrcii.ru
ORCID iD: 0000-0003-4961-9640
SPIN-code: 3199-9803

MD, Dr. Sci. (Med.), Professor, Corresponding member of the Russian Academy of Sciences

Russian Federation, Moscow; Moscow

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Supplementary files

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2. Fig. 1. Growth intensity of E. coli Dh5α colonies under the influence of AB-18.

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3. Fig. 2. Growth intensity of E. coli Dh5α colonies under the influence of AB-17.

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4. Fig. 3. Growth intensity of E. coli Dh5α colonies under the influence of AB-14.

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