Assessment of cytokine profile and T-lymphocyte subpopulations in blood serum in children with allergic diseases of the respiratory system

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Abstract


Expansion of the range of examined T-helper clones has determined more complex immune mechanisms for the implementation of allergic inflammation. Objective. To characterize the parameters and relationships between the serum cytokine profile and T-lympho-cyte subpopulation in peripheral blood of children with bronchial asthma and allergic rhinitis. Materials and methods. 150 children aged between 3-11 years old with bronchial asthma, and allergic rhinitis and 30 healthy volunteers were examined. Immunological parameters were assessed by flow cytometry, the concentration of serum interleukins and IgE were determined by means of enzymelinked immunosorbent assay. Statistical analysis was performed with «Statistica 10» program with a critical level of significance p<0.05. Results. High levels of interleukins -4, -8, -13, 17A were determined, IL-7F level was not significantly different from that in control group and low level of IFN-y was found in the serum of children with allergic diseases. The number of CD3+CD8+CD45RO+, CD3+CD8+CD45RACD45RO+ T-lymphocytes, CD3+CD4+ T-helper cells and Th17 were increased and at the same time CD3+CD4+CD45RO+ memory cells were decreased In bronchial asthma and allergic rhinitis children. Number of Th17 cells in healthy children was 9.49±1.6%, in allergic children it was significantly higher - 14.5±0.77% (p<0.001). Analyses of serum cytokine count in children with isolated BA and in association with allergic rhinitis revealed multidirectional correlations differing in strength and direction from those in the group of healthy children. Conclusion. In children with isolated bronchial asthma and associated with allergic rhinitis the following parameters were found: CD3+CD4+ T-cells count was comparable to that in healthy children, the imbalance of T-helper subpopulation: prevalence of Th2 and Th17, activation of IL-17A, IL-4, IL-8, IL-13 synthesis and low level of serum IFN-y.

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E V Prosekova

Federal State Budgetary Educational Institution of Higher Education «Pacific State Medical University» of the Ministry of Healthcare of the Russian Federation

Email: pros.ev@mail.ru

A I Turyanskaya

Federal State Budgetary Educational Institution of Higher Education «Pacific State Medical University» of the Ministry of Healthcare of the Russian Federation

N G Plekhova

Federal State Budgetary Educational Institution of Higher Education «Pacific State Medical University» of the Ministry of Healthcare of the Russian Federation

M S Dolgopolov

Federal State Budgetary Educational Institution of Higher Education «Pacific State Medical University» of the Ministry of Healthcare of the Russian Federation

V A Sabynych

Federal State Budgetary Educational Institution of Higher Education «Pacific State Medical University» of the Ministry of Healthcare of the Russian Federation

  1. Global strategy for asthma management and prevention. Global Initiative for Asthma. (GINA) 2018. Available from: www.ginasthma.org.
  2. Brozek JL, Bousquet J., Agache I., Agarwal A., Bachert C., Bosnic-Anticevich S. Allergic Rhinitis and its Impact on Asthma (ARIA) guidelines-2016 revision. J. Allergy Clin Immunol. 2017;140:950-958. doi: 10.1016/j.jaci.2017.03.050.
  3. Agache I., Akdis CA, Chivato T., Hellings P., Hoffman-Sommergruber K., Jutel M. et al. EAACI White Paper on Research, Innovation and Quality Care Published by the European Academy of Allergy and Clinical Immunology. Zurich. Switzerland. 2018:152.
  4. Kubo M. Innate and adaptive type 2 immunity in lung allergic inflammation. Immunol Rev. 2017;278:162-172.
  5. Cosmi L., Liotta F., Maggi L., Annunziato F. Role of Type 2 Innate Lymphoid Cells in Allergic Diseases. Curr Allergy Asthma Rep. 2017;17:66. doi: 10.1007/s11882-017-0735-9.
  6. Varricchi G., Harker J., Borriello F., Marone G., Durham SR, Shamji MH. T. follicular helper (Tfh) cells in normal immune responses and in allergic disorders. Allergy 2016;71:1086-1094. doi: 10.1111/all.12878.
  7. Козлов ВА. Иммунная парадигма и иммуносупрессорная доминанта в патогенезе основных заболеваний современного человека. Бюллетень сибирской медицины. 2019;18(1):7-17
  8. Eberle G. Immunity by equilibrium. Nature Revieus. Immunology. 2016;16:524-532.
  9. Lambrecht BN, Hammad H. The immunology of asthma. Nature Immunology. 2015;16 (1):45-56. doi: 10.1038/ni.3049.
  10. Курбачёва ОМ, Жестков АВ, Нагаткин ДА, Кулагина ВВ, Нагаткина ОВ. Современный взгляд на иммунопатогенез бронхиальной астмы. Российский Аллергологический Журнал. 2016;2:10-14
  11. Muehling LM, Lawrence MG, Woodfolk JA. Pathogenic CD4+ T-cells in patients with asthma. J. Allergy Clin Immunol. 2017;140(6):1523-1540. doi: 10.1016/j.jaci.2017.02.025.
  12. Acosta-Rodriguez E. V., Napolitani G., Lanzavecchia A., Sallusto F. Interleukins 1 p and 6 but not transforming growth factor - p are essential for the differentiation оf interleukin-17 - producing human T-helper cells. Nature immunol. 2007;8(9):942-949.
  13. Willem van de V., Mubeccel A. Mechanisms of immune regulation in allergy. Global Atlas of allergy. 2014;90-91. doi: 10.1111/imr.12555.
  14. Кудрявцев ИВ, Борисов АГ, Волков АЕ, Савченко АА, Серебрякова МК, Полевщиков АВ. Анализ уровня экспрессии CD56 и CD57 цитотоксическими Т-лимфоцитами различного уровня дифференцировки. Тихоок. мед. журн. 2015;2:30-35
  15. Lund FE, Randall TD. Effector and regulatory B-cells: modulators of CD4+ T-cell immunity. Nat Rev Immunol. 2010;10:236-247. doi: 10.1038/nri2729.
  16. Akdis M. Healthy immune response to allergens: T-regulatory cells and more. Curr Opin Immunol. 2006;18:738-744.
  17. Симбирцев АС. Цитокины в патогенезе и лечении заболеваний человека. СПб.: Фолиант. 2018:512
  18. Eusebio M., Kuna P., Kraszula L., Kurczyk M., Pietruczuk M. Allergy-related changes in levels of CD8+CD25+Foxp3 (bright) Treg cells and Foxp3 mRNA expressionin peripheral blood: the role of IL-10 or TGF-p. J. Biol Regul Homeost Agents. 2014;28(3):461-470.
  19. Agache I., Akdis CA. Endotypes of allergic diseases and asthma: An important step in building blocks for the future of precision medicine. Allergol Int. 2016;65:243-252. DOI: 10.1016/j. alit.2016.04.011.
  20. Национальная программа «Бронхиальная астма у детей. Стратегия лечения и профилактика». Москва: Оригинал-макет. 2017:160
  21. Bousquet J., Pfaar O., Togias A., Schunemann HJ, Ansotegui I., Papadopoulos NG et al. 2019 Allergic Rhinitis and its Impact on Asthma (ARIA). Care pathways for allergen immunotherapy. Allergy. 2019. doi: 10.1111/all.13805.
  22. Хайдуков СВ, Байдун ЛА, Зурочка АВ, Тотолян АА. Стандартизованная технология «Исследование субпо-пуляционного состава лимфоцитов периферической крови с применением проточных цитофлюориметров-анализаторов». Российский иммунологический журнал. 2014;8(4):974-992
  23. Bossowski A., Moniuszko M., Idzkowska E., Dqbrows-ka M., Jeznach M., Sawicka B. et al. Evaluation of CD4+ CD161+CD196+ and CD4+IL-17+ Th17 cells in the peripheral blood of young patients with Hashimoto’s thyroiditis and Graves’ disease. Pediatr Endocrinol Diabetes Metab. 2012;18(3):89-95.
  24. Mahnke YD, Roederer M. Optimizing a multicolor immuno-phenotyping assay. Clin Lab Med. 2007;27(3):469-485.
  25. Zhao Y., Yang J., Gao Y. Altered expressions of helper T-cell (Th)1, Th2, and Th17 cytokines in CD8+ and yS T-cells in patients with allergic asthma. J. Asthma. 2011;48:429-436. DO I: 10.3109/02770903.2011.570403.
  26. Liu MC, Proud D., Lichtenstein LM, Hubbard WC, Bochner BS, Stealey BA et al. Effects of prednisone on the cellular responses and release of cytokines and mediators after segmental allergen challenge of asthmatic subjects. J. Allergy Clin Immunol. 2001;108:29-38.
  27. Francis JN, Sabroe I., Lloyd CM, Durham SR, Till SJ. Elevated CCR6+CD4+ T-lymphocytes in tissue compared with blood and induction of CCL20 during the asthmatic late response. Clin Exp Immunol. 2008;152(3):440-447. doi: 10.1111/j.1365-2249.2008.03657.x.

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