The species differences of staphylococci isolated from skin lesions in children and adolescents with atopic dermatitis



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Abstract

Materials and methods. This study enrolled 87 patients with AD different manifestations in its acute phase. Most children were with moderate AD. In over 50% of cases we detected the erythemato-squamous form of AD (ES). 9 patients with severe manifestations had a lichenoid form. Skin smears were taken before starting therapy. The identification of staphylococcal types was performed and their antibiotic sensitivity was tested by means of the automated microbiology analyzer «Walka-way 96 plus», Siemens Healthcare Diagnostics, Germany. Results. The study proved that S. aureus and S. epidermidis (SA and SE) were mostly isolated from skin lesion: the coagulazo-negative staphylococci (CNS) included S. haemolyticus, S. warneri, S. hominis, S. saprophyticus and S. sciuri. 12,7% cases showed no bacterial growth. No difference between SA or CNS and the disease severity was recorded. CNS were proved to colonize more frequently the skin of patients with erythemato-squamous AD while SA was a more frequent colonizer in chronic AD with lichenization (р=0,081). All staphylococci were penicillin-resistant. Among CNS, 8 out of31 SE strains, 1 S. haemolyticus and 1 S. sciuri were oxacillin-resistant. Conclusion. Therefore, we can state that alongside with SA colonization and regardless of AD severity CNS skin lesion colonization is detected in patients with AD. Moreover, CNS colonization accompanies acute inflammatory changes and in some cases demonstrates oxacillin-resistance. These findings suggest the need for further research into the role that CNS play in AD progression.

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About the authors

A V Kudryavtseva

I.M. Sechenov first Moscow State Medical University

Email: scientist002@yahoo.com
Pediatrics Hospital

J A Savvina

I.M. Sechenov first Moscow State Medical University

Pediatrics Hospital

O A Morozova

I.M. Sechenov first Moscow State Medical University

Pediatrics Hospital

References

  1. Данилычева И.В., Ильина Н.И. Качество жизни у больных крапивницей и атопическим дерматитом. Consilium medicum. 2001, № 3, с. 184-186.
  2. Alzolibani A.A. Impact of atopic dermatitis on the quality of life of Saudi children. Saudi Med. J. 2014, v. 35, p. 391-396.
  3. Leung D.Y.M. The role of Staphylococcus aureus in atopic eczema. Acta Derm. Venereol. 2008, v. 216, р. 21-27.
  4. Ong P. Y., Ohtake T., Brandt C. et al. Endogenous antimicrobial peptides and skin infections in atopic dermatitis. N. Engl. J. Med. 2002, v. 347, р. 1151-1160.
  5. Mempel M., Schmidt T., Weidinger S. et al. Role of Staphylococcus aureus surface-associated proteins in the attachment to cultured HaCaT keratinocytes in a new adhesion assay. J. Invest. Dermatol. 1998, v. 111, р. 452-456.
  6. Nomura I., Goleva E., Howell M.D. et al. Cytokine milieu of atopic dermatitis, as compared to psoriasis, skin prevents induction of innate immune response genes. J. Immunol. 2003, v. 171, р. 3262-3269.
  7. Lomholt H., Andersen K.E., Kilian M. Staphylococcus aureus Clonal Dynamics and Virulence Factors in Children with Atopic Dermatitis. J. Invest. Dermatol. 2005, v. 125, р. 977-982.
  8. Neuber K., Konig W. Effects of Staphylococcus aureus cell products (teichoic acid, peptidoglycan) and enterotoxin B on immunoglobulin (IgE, IgA, IgG) synthesis and CD23 expression in patients with atopic dermatitis. Immunol. 1992, v. 75, р. 23-28.
  9. Zollner T.M., Wichelhaus T.A., Hartung A. et al. Colonization with superantigen producting Staphylococcus aureus associated with increased severity of atopic dermatitis. Clin. Exp. Allergy. 2000, v. 30, р. 994-1000.
  10. Breuer K., Wittmann M., Bosche B. et al. Severe atopic dermatitis is associated with sensitization to staphylococcal enterotoxin B (SEB). Allergy. 2000, v. 55, р. 551-555.
  11. Arkwright P.D., Cookson B.D. Children with atopic dermatitis who carry toxin-positive Staphylococcus aureus strains have an expansion of blood CD-5-Blymphocytes without an increase in disease severity. Clin. Exp. Immunol. 2001, v. 125, р. 184-189.
  12. Soares J., Lopes C., Tavaria F. et al. A diversity profile from the staphylococcal community on atopic dermatitis skin: a molecular approach. J. Appl. Microbiol. 2013, v. 115, р. 1411-1419.
  13. Флуер Ф.С., Кудрявцева А.В., Морозова О.А., Максимушкин А.Ю. Энтеротоксигенная активность разных видов стафилококков, выделенных при атопическом дерматите у детей. Аллергология и иммунология в педиатрии. 2012, № 4, с. 8-10.
  14. Hanifin J.M., Rajka G. Diagnostic features of atopic dermatitis. Acta Derm. Venerol. 1980, v. 92, р. 44-47.
  15. Балаболкин И.И., Гребенюк Н.В. Атопический дерматит у детей. М., «Медицина». 1999, 240 с.
  16. Gong J.Q., Lin L., Lin T et al. Skin colonization by Staphylococcus aureus in patients with eczema and atopic dermatitis and relevant combined topical therapy: a double-blind multicentre randomized controlled trial. Br. J.Dermatol. 2006, v. 155, p. 680-687.
  17. Кудрявцева А.В., Флуер Ф.С., Балаболкин И.И. и соавт. Зависимость тяжести течения атопического дерматита у детей от токсинпродуцирующих свойств штаммов золотистого стафилококка. Рос. педиатр. журн. 2009, № 3, с. 31-37.
  18. Park H.Y., Kim C.R., Huh I.S. et al. Staphylococcus aureus Colonization in Acute and Chronic Skin Lesions of Patients with Atopic Dermatitis. Ann. Dermatol. 2013, v. 25, p. 410-416.
  19. Jagadeesan S., Kurien G., Divakaran M.V. et al. Methicillin-resistant Staphylococcus aureus colonization and disease severity in atopic dermatitis: a cross-sectional study from South India. Indian J. Dermatol. Venereol. Leprol. 2014, v. 80, p. 229-234.
  20. Petry V., Lipnharski C., Bessa G.R. et al. Prevalence of community-acquired methicillin-resistant Staphylococcus aureus and antibiotic resistance in patients with atopic dermatitis in Porto Alegre. Brazil. Int. J. Dermatol. 2014, v. 53, p. 731-735.
  21. Мурашкин Н.Н., Глузмин М.И., Скобликов Н.Э. и соавт. Роль метициллинрезистентных штаммов золотистого стафилококка в патогенезе тяжелых форм атопического дерматита в детском возрасте. Пути достижения ремиссии. Вестник дерматол. и венерол. 2012, № 1, с. 66-74.
  22. Фассахов P.C. Антибиотикорезистентность Staphylococcus aureus, колонизирующего кожу и кишечник у больных атопическим дерматитом. Практическая медицина. 2009, № 3, с. 32-35.
  23. Тренева М.С., Пампура А.Н., Окунева Т.С. Атопический дерматит у детей: наличие специфических антител к суперантигенам Staphylococcus aureus и его антибиотикорезистентность. Педиатр. фармакол. 2012, № 3, с. 68-71.
  24. Воронина В.Р., Феденко Е.С., Пампура А.Н. Влияние колонизации кожи стафилококком и выделяемых им суперантигенов на течение атопического дерматита у детей. Рос. Аллерголог. Журн. 2004, № 3, с. 36-42.
  25. Tadayuki I., Yoshio U., Hitomi S., Akiko T. Staphylococcus epidermidis esp inhibits Staphylococcus aureus biofilm formation and nasal colonization. Nature. 2010, v. 465, p. 346-349.
  26. Frank D.N., Feazel L.M., Bessesen M.T. et al. The human nasal microbiota and Staphylococcus aureus carriage. PLoSONE. 2010, v. 5, e10598 pubmed.
  27. Кудрявцева А.В., Морозова О.А. Колонизация стафилококком кожных покровов детей с атопическим дерматитом как критерий эффективности наружного лечения. Практическая медицина. 2012, № 9, c. 279-283.
  28. Alsterholm M., Flytström I., Bergbrant I.M., Faergemann J. Fusidic acid-resistant Staphylococcus aureus in impetigo contagiosa and secondarily infected atopic dermatitis. Acta Derm. Venereol. 2010, v. 90, p. 52-57.

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