The species differences of staphylococci isolated from skin lesions in children and adolescents with atopic dermatitis



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Abstract

Materials and methods. This study enrolled 87 patients with AD different manifestations in its acute phase. Most children were with moderate AD. In over 50% of cases we detected the erythemato-squamous form of AD (ES). 9 patients with severe manifestations had a lichenoid form. Skin smears were taken before starting therapy. The identification of staphylococcal types was performed and their antibiotic sensitivity was tested by means of the automated microbiology analyzer «Walka-way 96 plus», Siemens Healthcare Diagnostics, Germany. Results. The study proved that S. aureus and S. epidermidis (SA and SE) were mostly isolated from skin lesion: the coagulazo-negative staphylococci (CNS) included S. haemolyticus, S. warneri, S. hominis, S. saprophyticus and S. sciuri. 12,7% cases showed no bacterial growth. No difference between SA or CNS and the disease severity was recorded. CNS were proved to colonize more frequently the skin of patients with erythemato-squamous AD while SA was a more frequent colonizer in chronic AD with lichenization (р=0,081). All staphylococci were penicillin-resistant. Among CNS, 8 out of31 SE strains, 1 S. haemolyticus and 1 S. sciuri were oxacillin-resistant. Conclusion. Therefore, we can state that alongside with SA colonization and regardless of AD severity CNS skin lesion colonization is detected in patients with AD. Moreover, CNS colonization accompanies acute inflammatory changes and in some cases demonstrates oxacillin-resistance. These findings suggest the need for further research into the role that CNS play in AD progression.

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About the authors

A V Kudryavtseva

I.M. Sechenov first Moscow State Medical University

Email: scientist002@yahoo.com
Pediatrics Hospital

J A Savvina

I.M. Sechenov first Moscow State Medical University

Pediatrics Hospital

O A Morozova

I.M. Sechenov first Moscow State Medical University

Pediatrics Hospital

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