Suppression spontaneous and induced expression of CD203c basophils after subcutaneous immunotherapy with ragweed and mugwort allergens
- Authors: Barycheva L.Y.1, Dushina L.V.1,2, Masalskiy S.S.3,4, Smolkin Y.S.3,4,5, Kozmova N.A.1, Kuropatnikova E.A.4,5
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Affiliations:
- Stavropol State Medical University
- Stavropol Regional Clinical Consultative and Diagnostic Center
- Association Pediatric Allergist and Immunologist Russia
- Scientific-Clinical Consultative Center of Allergology and Immunology
- Academy of Postgraduate Education of the Federal Research and Clinical Center of Specialized Medical Care and Medical Technologies
- Issue: Vol 19, No 3 (2022)
- Pages: 299-316
- Section: Original studies
- URL: https://rusalljournal.ru/raj/article/view/1534
- DOI: https://doi.org/10.36691/RJA1534
- ID: 1534
Cite item
Abstract
BACKGROUND: The basophil activation test (BAT) reflects the presence of immunoglobulin E on the cell surface and can be a tool for monitoring the effectiveness of allergen-specific immunotherapy. Spontaneous and allergen-induced BAT levels may likely change after effective subcutaneous immunotherapy, and this is of clinical importance.
AIMS: To determine the basophil activation markers in patients sensitized to weed pollen during subcutaneous immunotherapy
MATERIALS AND METHODS: This clinical study of 2 years included 60 patients with allergic rhinitis who underwent subcutaneous immunotherapy with weed allergens for two seasons. The proportion of basophil-expressing CD203c, spontaneously and after exposure to a trigger allergen, was determined. BAT was performed before treatment and after the first and second courses of preseason subcutaneous immunotherapy (0, 3, and 15 months of treatment). The effectiveness of subcutaneous immunotherapy was assessed by the dynamics of the symptom scale (Rhinoconjunctivitis total symptom score [RTSS]) and the drug assessment (Daily medical score [DMS]).
RESULTS: Subcutaneous immunotherapy with water–salt extracts was effective. The median RTSS in patients after two courses decreased from 13.0 [10.0; 15.0] to 4.5 [3.00; 7.25] points (p <0.001) and DMS score from 2.0 [2.0; 2.0] to 1.0 [1.0; 2.0] points (p <0.001). Spontaneous BAT remained unchanged after the first course of allergen-specific immunotherapy. The mean BAT values before therapy and after the first course of subcutaneous immunotherapy did not differ (8.15% [6.10; 11.9] vs. 9.0% [6.30; 12.0]). The spontaneous BAT decreased after the second course of treatment (8.15% [6.10; 11.9] vs. 6.20% [4.27; 9.00], χ2=15.5, p <0.001). Allergen-induced BAT significantly decreased from the first year of treatment. The overall change in the total group for BAT with ragweed after the first course of subcutaneous immunotherapy was 11.25% (95% confidence interval [CI]: 7.10–15.10, p <0.001). The overall difference in the induced ВAT test at the end of therapy reached 8.63% (95% CI: 5.30–12.05, p <0.001). The indices of induced BAT decreased by 12.0% after the first course of therapy with mugwort allergens (95% CI: 6.95–17.90, p <0.001), and the difference from the initial values after the second course was 9.67% (95% CI: 6.85–15.90, p <0.001).
CONCLUSIONS: Subcutaneous immunotherapy with ragweed and mugwort allergens was effective. The change in spontaneous and induced BAT is associated with a positive effect of allergen-specific immunotherapy.
Full Text

About the authors
Liudmila Yu. Barycheva
Stavropol State Medical University
Author for correspondence.
Email: for_ludmila@inbox.ru
ORCID iD: 0000-0002-4069-0566
SPIN-code: 8946-3393
MD, Dr. Sci. (Med.), Professor
Russian Federation, StavropolLiudmila V. Dushina
Stavropol State Medical University; Stavropol Regional Clinical Consultative and Diagnostic Center
Email: dushina.stv@gmail.com
ORCID iD: 0000-0001-5806-972X
SPIN-code: 3599-5095
MD, Cand. Sci. (Med.)
Russian Federation, Stavropol; StavropolSergey S. Masalskiy
Association Pediatric Allergist and Immunologist Russia; Scientific-Clinical Consultative Center of Allergology and Immunology
Email: masalsky85@gmail.com
ORCID iD: 0000-0002-2048-5709
SPIN-code: 1709-2182
MD, Cand. Sci. (Med.)
Russian Federation, Moscow; MoscowYuri S. Smolkin
Association Pediatric Allergist and Immunologist Russia; Scientific-Clinical Consultative Center of Allergology and Immunology; Academy of Postgraduate Education of the Federal Research and Clinical Center of Specialized Medical Care and Medical Technologies
Email: smolking@df.ru
ORCID iD: 0000-0001-7876-6258
SPIN-code: 7068-4939
MD, Dr. Sci. (Med.)
Russian Federation, Moscow; Moscow; MoscowNatalia A. Kozmova
Stavropol State Medical University
Email: kozmova@mail.ru
ORCID iD: 0000-0003-0971-5347
SPIN-code: 5236-9634
Russian Federation, Stavropol
Elena A. Kuropatnikova
Scientific-Clinical Consultative Center of Allergology and Immunology; Academy of Postgraduate Education of the Federal Research and Clinical Center of Specialized Medical Care and Medical Technologies
Email: Lenysionok@mail.ru
ORCID iD: 0000-0001-5764-6441
SPIN-code: 2065-9023
Russian Federation, Moscow; Moscow
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