Polymorphic variants of matrix metalloproteinases genes in children with atopic dermatitis

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Abstract


Matrix metalloproteinases (MMP) play a special role in the pathogenesis of atopic dermatitis (AD). Therefore, the study of the features of genes responsible for the synthesis of MMP in children with AD is of great scientific and practical interest. Background. To study the role of polymorphic variants of matrix metalloproteinase genes (MMP 9 and MMP20) in the pathogenesis of AD in children. Materials and methods. Allelic variants of320A>C gene MMP20,837T>C gene MMP20, -8202 A>G gene MMP9 in children with AD were studied using the method of allele-specific polymerase chain reaction. The control group consisted of I and IIa the health groups patients of the corresponding sex and age. Results. The results of genetic studies showed that the incidence of alleles and genotypes in the polymorphisms 320A>C of the gene MMP20, 837T>C of the gene MMP20 in patients had no significant differences from the control group (p>0.05). It was established that the A/A-genotype of polymorphism -8202 A>G of the MMP9 gene, prevailed among children suffering from AD at a frequency of 69.2%, whereas in the group of healthy children the frequency of this genotype was 3 times lower (p=0.003). At the same time A/G-genotype (55.7%) was predominant in the control group, while G/G genotype was 2 times lower (21.3%). Thus the risk of AD increased by 7.55 times (OR=7.55 [95% Cl - 2.97-19.21; p<0.001] in children with genotype A/A. Conclusion. The most significant polymorphism in the pathogenesis of AD in children is 8202 A>G of the MMP9 gene, in particular the risk of developing of skin manifestations of allergy is increased by more than 7 times in A-allele homozygotes.

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O E Semernik

Rostov State Medical University

Email: sememick@mail.ru

A A Lebedenko

Rostov State Medical University

T P Shkurat

Southern Federal University

E V Mashkina

Southern Federal University

T K Dreyzina

Southern Federal University

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