Email this article 
No metabolites impact in allergic inflammation regulation and their contribution to remodelling bronchi mucous in bronchial asthma patients
- Authors: Ogorodova LM1, Kozina OV1, Gereng EA1, Ogorodova LM1, Kozina OV1, Gereng EA1
-
Affiliations:
- Issue: Vol 6, No 5 (2009)
- Pages: 11-17
- Section: Articles
- URL: https://rusalljournal.ru/raj/article/view/1025
- DOI: https://doi.org/10.36691/RJA1025
- ID: 1025
Cite item
Open Access
Access granted
Subscription or Fee Access
Abstract
Material and methods. Morphological and morphometrical methods of investigation of bronchi mucous of 39 BA patients were used, IL-4, TNF-α, INF-g, IL-8, nitrite, 3-nitrotyrosine, nitrosoglutathion levels in bronchoalveolar washout were investigated, nitrite concentration in condensate of the exhaled air was measured. Results. The increased level of IL-4, TNF-α, INF-g, IL-8 in bronchoalveolar washout is associated with persistent Th2-inflammation. The BA severity correlate with highest IL-8, TNF-a, INF-g, nitrite and 3-nitrotyrosine level but with nitrosoglutathion deficit and in general is associated with structured architectonics bronchi mucous abnormalities.
Conclusion. The association of toxic NO metabolites, pro-inflammation cytokines levels, bronchi mucous path-omorphological abnormalities with clinical signs of BA confirms the role of toxic NO metabolites in development of Th2-inflammations.
About the authors
L M Ogorodova
O V Kozina
Email: ovkozina2006@rambler.ru
E A Gereng
L M Ogorodova
O V Kozina
E A Gereng
References
- Global Initiative for Asthma. NIH Publication number. 01-3659, NHLBI/WHO 2006. National Institutes of Health.
- Ricciardolo F.M., Sterk P.J., Gaston B. and Folkerts G. Nitric Oxide in Health and Disease of the Respiratory System. Physiol. 2004,v. 84, p. 731-765.
- Bove P.F., Vliet A. Nitric oxide and reactive nitrogen species in airway epithelial signaling and inflammation. Free Radic. Biol. and Med. 2006, v. 41 (4), p. 515-527.
- Giustarini D., Milzani A., Colombo R. et al. Nitric oxide and S-nitrosothiols in human blood. Clin. Chim. Acta 2003, v. 330 (1-2), p. 85-98.
- Truyen E., Coteur L., Dilissen E. Evaluation of airway inflammation by quantitative Th1/Th2 cytokine mRNA measurement in sputum of asthma patients. Thorax. 2006, v. 61 (3), p. 202-208.
- Romanska H.M., Polak J.M., Coleman R.A. et al. iNOS gene upregulation is associated with the early proliferative response of human lung fibroblasts to cytokine stimulation. J Pathol. 2002, v. 197, p. 372-379.
- Каминская Л.Ю., Жлоба А.А., Александрова Л.А. и др. Влияние донатора NO нитрозотиола глутатиона на уровень окислов азота и малонового диальдегида в крови крыс. Артериальная гипертензия. 2005, № 1, с. 10-17.
- Massey R.C., Dennis M.J., McWeeny D.J. Some observations concerning the S-nitroso and S-phenylsulphonyl derivatives of L-cysteine and glutathione. Tetrahedron Letters. 1985, v. 26 (16), p. 2013-2016.
- Barnes P.J. The role inflammation and anti-inflamatory medication in asthma. Respir. Med. 2002, v. 96, p. 9-15.
- Козина О.В., Огородова Л.М., Андрушкевич В.В. и соавт. Метаболиты оксида азота и их значение в патогенезе бронхиальной астмы. Клин. и лаб. диагн. 2008, №. 6, с. 34-37.
- Козина О.В., Андрушкевич В.В., Сазонов А.Э. и соавт. Клинико-биохимические аспекты развития обструкции бронхов при бронхиальной астме. Пульмонология. 2008, № 2, с. 52-57.
- Silvestri M., Bontempelli M., Giacomelli M. et al. High serum levels of tumour necrosis factor-a and interleukin-8 in severe asthma: markers of systemic inflammation? Clinic. Exp. Allergy. 2006, v. 36 (11), p. 1373-1381.
- Celio S., Troxler H., Durka S.S. et al. Free 3-nitrotyrosine in exhaled breath condensates of children fails as a marker for oxidative stress in stable cystic fibrosis and asthma. Nitric Oxide. 2006, v. 15 (3), p. 226-232.
- Старикова Э.А., Фрейдлин И.С., Соколов Д.И., Сельков С.А. Изменения свойств эндотелиальных клеток линии ЕА.hy 926 под влиянием фактора некроза опухоли α, интерферона-γ и интерлейкина-4. Иммунология. 2005, №. 2, с. 83-87.
Supplementary files
