Study of antiallergic properties and toxicity of aqueous dispersion of fullerene C60 on laboratory animals



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Abstract

AIMS: To evaluate the antiallergic activity of an aqueous dispersion of fullerene C60 (ADF) and its safety in preclinical studies.

MATERIALS AND METHODS: Food allergy was induced in BALB/c mice by ovalbumin. ADF was administered intragastrically in different regimens. Specific IgE, Th2 cytokines, body weight, diarrhea, and jejunal histology were assessed. Allergenicity was tested in mice and guinea pigs using standard hypersensitivity assays, and immunotoxicity was evaluated in humoral, cellular, and phagocytic immunity models.

RESULTS: ADF reduced OVA-specific IgE, IL-4 and IL-5, alleviated diarrhea, and supported weight gain. Histology showed decreased eosinophilia in the jejunum. No allergenic or immunotoxic effects were detected in preclinical tests.

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About the authors

Renata A. Kurmasheva

Государственный научный центр «Институт иммунологии»

Author for correspondence.
Email: renata.adilevna@yandex.ru
ORCID iD: 0009-0003-8703-5686
SPIN-code: 2321-4718
Москва

Nadezda N. Shershakova

National Research Center — Institute of Immunology Federal Medico-Biological Agency

Email: nn.shershakova@nrcii.ru
ORCID iD: 0000-0001-6444-6499
SPIN-code: 7555-5925

MD, Dr. Sci. (Biology)

Russian Federation, Moscow

Larisa V. Mikhina

Research Center for Toxicology and Hygienic Regulation of Biological Products — Branch of the National Research Center — Institute of Immunology Federal Medico-Biological Agency

Email: larisa_mihina@mail.ru
ORCID iD: 0000-0003-4931-5380
SPIN-code: 4040-9486

MD, Cand. Sci. (Biology)

Russian Federation, Serpukhov

Elena N. Baraboshkina

National Research Center — Institute of Immunology Federal Medico-Biological Agency

Email: en.baraboshkina@nrcii.ru
ORCID iD: 0009-0002-0981-5540
SPIN-code: 3080-0926

MD, Cand. Sci. (Biology)

Russian Federation, Moscow

Larisa A. Eremenko

Email: laeremenko@yandex.ru
SPIN-code: 7054-2236

Elena I. Sokolova

Email: el.kuks@yandex.ru

Evgeny A. Turetskiy

NRC Institute of Immunology FMBA of Russia

Email: EA.Turetskiy@nrcii.ru
ORCID iD: 0000-0002-6822-3409

Junior Researcher, Laboratory of Peptide Immunogens

Russian Federation, 24, Kashirskoye shosse, Moscow, 115522

Denis S. Gorshenin

National Research Center — Institute of Immunology Federal Medico-Biological Agency

Email: dgorhenin63@gmail.com
ORCID iD: 0000-0002-1926-6090
SPIN-code: 5426-1810
Russian Federation, Moscow

Oleg Yu. Kamishnikov

Email: raddabayoleg@gmail.com
SPIN-code: 1467-9262

Sergei M. Andreev

National Research Center – Institute of Immunology Federal Medical-Biological Agency of Russia

Email: andsergej@yandex.ru
ORCID iD: 0000-0001-8297-579X
SPIN-code: 2542-5260

Cand. Sci. (Chemistry)

Russian Federation, Moscow

Nikolay M. Onatsky

Research Center for Toxicology and Hygienic Regulation of Biological Products — Branch of the National Research Center — Institute of Immunology Federal Medico-Biological Agency

Email: onatsky@toxicbio.ru
ORCID iD: 0009-0001-4826-7092
SPIN-code: 9404-4895

MD, Cand. Sci. (Biology)

Russian Federation, Serpukhov

Musa R. Khaitov

National Research Center — Institute of Immunology Federal Medico-Biological Agency; The Russian National Research Medical University named after N.I. Pirogov

Email: mr.khaitov@nrcii.ru
ORCID iD: 0000-0003-4961-9640
SPIN-code: 3199-9803

MD, Dr. Sci. (Medicine), Professor, corresponding member of Russian Academy of Sciences

Russian Federation, Moscow; Moscow

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