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Assessment of antimicrobial peptides(alpha-defensins) in atopic dermatitis and pyodermia patients blood
- Authors: Tsyvkina EA1, Fedenko ES1, Budikhina AS1, Pinegin BV1
-
Affiliations:
- Institute of Immunology
- Issue: Vol 7, No 5 (2010)
- Pages: 43-47
- Section: Articles
- URL: https://rusalljournal.ru/raj/article/view/887
- DOI: https://doi.org/10.36691/RJA887
- ID: 887
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Abstract
The purpose of research. The purpose was to investigate alpha-defensin levels in the circularly neutrophiles of atopic dermatitis and pyodermia patients in comparison with healthy donors.
Materials and methods. 27 patients with atopic dermatitis, 31 patients with pyodermia in comparison with 17 healthy donors were investigated. Intracellular alpha-defensin levels in neutrophils in the peripheral blood were estimated by flow cytometry with mouse anti-HNP-antibodies (Hy cult biotechnology).
Results. The alpha-defensins level in patients with atopic dermatitis and pyodermia was reduced in comparison with healthy donors. More expressed decrease of alpha-defensins level was obtained in patients with pyodermia and sever atopic dermatitis with skin infection.
Conclusion. Thus, a decrease of alpha-defensins levels in atopic dermatitis and pyodermia patients, possibly, is a marker of the chronic bacterial inflammation and may cause chronic bacterial skin disease, St. aureus colonizations and tolerance to the therapy.
Materials and methods. 27 patients with atopic dermatitis, 31 patients with pyodermia in comparison with 17 healthy donors were investigated. Intracellular alpha-defensin levels in neutrophils in the peripheral blood were estimated by flow cytometry with mouse anti-HNP-antibodies (Hy cult biotechnology).
Results. The alpha-defensins level in patients with atopic dermatitis and pyodermia was reduced in comparison with healthy donors. More expressed decrease of alpha-defensins level was obtained in patients with pyodermia and sever atopic dermatitis with skin infection.
Conclusion. Thus, a decrease of alpha-defensins levels in atopic dermatitis and pyodermia patients, possibly, is a marker of the chronic bacterial inflammation and may cause chronic bacterial skin disease, St. aureus colonizations and tolerance to the therapy.
Keywords
About the authors
E A Tsyvkina
Institute of Immunology
Email: katia1982@inbox.ru
Institute of Immunology
E S Fedenko
Institute of ImmunologyInstitute of Immunology
A S Budikhina
Institute of ImmunologyInstitute of Immunology
B V Pinegin
Institute of ImmunologyInstitute of Immunology
References
- Richard L.Gallo, MD, PhD, Masamoto Murakami, MD, PhD, Takaaki Ohtake, MD, PhD et al. Biology and clinical relevance of naturally occurring antimicrobial peptides. J. allergy and clinical immunology. 2002, v. 110, No. 6, р. 823-831.
- Siegbert Rieg, Heiko Steffen, Silke Seeber et al. Deficiency of dermicidin-derived antimicrobial peptides in sweat of patients with atopic dermatitis correlates with an impaired innate defense of human skin in vivo. The journal of immunology. 2005, No. 174, p. 8003-8010.
- Будихина А.С. Оценка бактерицидной активности биологических жидкостей в норме и при патологии с помощью лазерной проточной цитометрии. Диссертация канд. мед. наук. М., 2007, 158 с.
- Будихина А.С., Пинегин Б.В. ß-Дефензины: свойства и функции. Рос. Аллергол. Журн. 2008, № 3, с. 15-21.
- Ong P.Y., Ohtake T., Brandt C. et al. Endogenous antimicrobial peptides and skin infections in atopic dermatitis. N. Eng. J. Med. 2002, v. 347, p. 1151-1160.
- Howell M.D. Boguniewicz M., Pastore S. et al. Mechanism of HBD-3 deficiency in atopic dermatitis. J. Clinical Immunology. 2006, v. 121, p. 332-338.
- Елисютина О.Г. Обоснование специфической иммунотерапии больных атопическим дерматитом, осложненным рецидивирующей пиодермией. Диссертация на соискание ученой степени канд. мед. наук. М., 2006, 165 с.
- Елисютина О.Г., Феденко Е.С. Роль S. aureus в патогенезе атопического дерматита. Рос. Аллергол. Журн. 2004, № 1, с. 17-21.
- Хамаганова И. В. Гнойничковые заболевания кожи. Леч. врач. 2006, № 9, с. 5-12.
- Сетдикова H.X., Латышева Т.В. Комплексные механизмы развития хронического рецидивирующего фурункулеза и пути их коррекции. Иммунология. 2000, № 3, с. 48-50
- Catherine M.T. Chronnell, Lucy R. Ghali, Rozina S. Ali et al. Human ß-Defensin-1 and -2 Expression in Human Piloseba-ceous Units: Upregulation in Acne Vulgaris Lesions. Journal of Investigative Dermatology, 2001, v. 117, р. 1120-1125.
- Yamasaki K., Gallo R.L. Antimicrobial peptides in human skin disease. Eur. J. Dermatol. 2008, v. 18 (1), p. 11-21.
- Philpott M. Defensins and acne. Mol. Immunol. 2003, v. 40 (7), p. 457-462.
- Hanifin J.M., Rajka G. Diagnostic features of atopic dermatitis. Acta Derm. Venerol. 1980, v. 92 (suppl), p. 44-47.
- Российский национальный согласительный документ по атопическому дерматиту. Под ред. Р.М. Хаитова, А.А. Кубановой. Атопический дерматит: рекомендации для практических врачей. М., «Фармарус принт». 2002, 192 с
- . Europian Task Force on Atopic Dermatitis. Severity scoring of atopic dermatitis: the SCORAD index. Dermatology. 1993, v. 186, p. 23-31.
- Дьяконова В.А., Пак В.Г., Будихина А.С. и соавт. Оценка функциональной активности фагоцитарной системы человека в норме и при патологии. Пособие для врачей клинической лабораторной диагностики. М., 2008, с. 29.
- Cole A.M. Antimicrobial peptide microbicides targeting HIV. Protein and Peptide Letters. 2005, v. 12, р. 41-47.
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