Topical immunotherapy influence on citokins genes expression in skin and blood in atopicdermatitis patients

Background. To study the clinical effectiveness of topical immune modulator cream thymogen 0,05% and its influence on some immunological parameters in the skin and blood of atopic dermatitis (AD) patients. Materials and methods. 40 people were included in the study, who were divided into main (AD patients, n=20) and a control group (healthy donors, n=20). All AD patients received topical treatment with cream thymogen 0,05% during 14 days. Patients applied cream to affected skin 2 times daily. Clinical efficacy of the treatment was assessed by changes of following parameters: investigated global assessment (IGA), subjective evaluation of the itch intensity and skin dryness . Samples of skin biopsies and peripheral blood of AD patients and healthy donors were tested for gene expression of cytokines by polymerase chain reaction in real time. Results. Topical treatment with cream thymogen 0,05% during 14 days in all AD patients showed positive clinical effect: reduction of dryness and itch. Immunological analysis revealed a significant increasing of cytokine IL-7 gene expression and decreasing of cytokines IL-8, IL-5 genes expression (p<0,05). In the peripheral blood no significant differences of cytokines genes expression before and after the treatment with cream thymogen 0,05% in comparison with healthy donors have been detected.
Conclusion. Cream thymogen 0,05% showed a local immunomodulating activity resulted in the decreasing of the intensity of skin inflammation and clinical improvement in AD patients. Changes of cytokines genes expression in skin, but not in blood were identified.

atopic dermatitis, immune response, skin biopsies, cytokines genes, topical immune modulators, thymogen