Improvement of control methods of treatment of mycoplasma infection in children with bronchial asthma



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Abstract

Background. Improvement of control methods of treatment of mycoplasma infection in children with bronchial asthma. Methods. 52 children from 1 to 4 years old with bronchial asthma who had mycoplasma antigens in the blood were examined before treatment of mycoplasma infection, 15 patients were examined in 1,5 months after treatment. Reaction of aggregate-hemagglutination, immunofluorescence assay and the reaction of the polymerase chain reaction (PCR) were used for detection of mycoplasmas’ antigens and DNA. Results. In reaction of aggregate-hemagglutination Mycoplasma pneumoniae (Mpn) was detected in 65,4% of patients, Mycoplasma hominis (Mh) — in 32,7%, Ureaplasma urealyticum — in 50%, Mycoplasma arthritidis — in 46,2%, Mycoplasma fermentrans — in 46,2%. In more detail, we investigated Mpn and Mh. Antigens of Mpn and Mh were detected in serum significantly frequently than DNA by PCR. DNA in structure of circulating immune complexes (CIC) was detected more often than in free state. After treatment by azithromycin the number of positive tests on antigens and DNA in free state and in structure of CIC decreased. In most cases DNA found in serum of blood in free state and in structure of CIC belongs to persistent living cells of mycoplasma. Conclusion. Empowering diagnosis of mycoplasma infections in asthma in children allows to base a comprehensive approach to laboratory diagnosis, monitor treatment, predict disease course.

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About the authors

N A Geppe

First Moscow State Medical University named after I.M. Sechenov (University Children's Hospital)

Moscow

N A Krylova

First Moscow State Medical University named after I.M. Sechenov (University Children's Hospital)

Email: nat.seliverstova@mail.ru
Moscow

I V Rakovskaia

FGBU NIIEM OF N.F. Gamalei behalf of the Health Ministry of Russia

Moscow

L G Gorina

FGBU NIIEM OF N.F. Gamalei behalf of the Health Ministry of Russia

Moscow

O I Barkhatova

FGBU NIIEM OF N.F. Gamalei behalf of the Health Ministry of Russia

Moscow

S A Goncharova

FGBU NIIEM OF N.F. Gamalei behalf of the Health Ministry of Russia

Moscow

G A Levina

FGBU NIIEM OF N.F. Gamalei behalf of the Health Ministry of Russia

Moscow

M G Utiusheva

First Moscow State Medical University named after I.M. Sechenov (University Children's Hospital)

Moscow

References

  1. Овсянников Д.Ю., Кузьменко Л.Г., Назарова Т.И. и соавт. Особенности бронхиальной астмы у детей, ассоциированной с микоплазменной и хламидийной инфекциями. Первый Конгресс педиатров-инфекционистов России. Актуальные вопросы инфекционной патологии у детей. Материалы. М., 2002, с. 141-142.
  2. Kraft M., Hamid Q. Mycoplasma in severe asthma. J. Allergy Clin. Immunol. 2006, v. 117 (5), p. 1197-1198.
  3. Waites K.B., Balish M.F., Atkinson T.P. New insights into the pathogenesis and detection of Mycoplasma pneumoniae infections. Future Microbiol. 2008, v. 3 (6), p. 635-648.
  4. Hassan J., Irwin F., Dooley S., Connell J. Mycoplasma pneumoniae infection in a pediatric population: analysis of soluble immune markers as risk factors for asthma. Hum. Immunol. 2008, v. 69 (12), p. 851-855.
  5. Atkinson T.P., Duffy L.B., Pendley D. et al. Deficient immune response to Mycoplasma pneumoniae in childhood asthma. Allergy Asthma Proc. 2009, v. 30 (2), p. 158-165.
  6. Ou C.Y., Tseng Y.F., Chiou Y.H. et al. The role of Mycoplasma pneumoniae in acute exacerbation of asthma in children. Acta Paediatr. Taiwan. 2008, v. 49 (1), p. 14-18.
  7. Кузьменко Л.Г., Корнюшин Р.А., Газарян Ж.Р., Киселева Н.М. Пульс-терапия азитромицином в комплексном лечении детей с бронхиальной астмой, ассоциированной с микоплазменной инфекцией. Вестник РУДН, серия «Медицина». 2003, № 5, c. 127.
  8. Горина Л.Г., Гончарова С.А., Раковская И.В., Кузьменко Л.Г. Частота обнаружения антигенов микоплазм в свободном состоянии и в составе циркулирующих иммунных комплексов у детей с бронхиальной астмой. Ж. микробиологии. 2006, № 4, с. 85-88.
  9. Горина Л.Г., Шершнёва Н.Н., Балабанов Д.Н. и соавт. Персистенция микоплазм при респираторных и урогенитальных инфекциях. Эпидемиология и вакцинопрофилактика. 2008, v. 6, с. 41-44.
  10. Zhang S., Wear D., Lo S. Mycoplasma infections alter gene expression in cultured human prostatic and cervical epithelial cells. FEMS Immunol. Med. Microbiol. 2000, v. 27 (1), p. 43-50.
  11. Whitcomb R.F. Methods in mycoplasmology. Academic Press, NY 1983, v. 1, p. 154-158.
  12. Кузьменко Л.Г., Тюрин Н.А., Брилькова Т.В. и соавт. Макролиды в комплексном лечении детей с бронхиальной астмой, ассоциированной с Mycoplasma pneumoniae. Вестник РУДН, серия «Медицина». 2002, № 4, с. 6-11.
  13. Sutherland E.R., Martin R.J. Asthma and atypical bacterial infection. Chest. 2007, v. 132 (6), p. 1962-1966.
  14. Hahn D.L. Is there a role for antibiotics in the treatment of asthma? Involvement of atypical organisms. BioDrugs. 2000, v. 14 (6), p. 349-354.
  15. Digeon M., Laver M., Riza J., Bach J. Defection of circulating immune complex in human sera by simplited assays with polyethylene glycol. J. Immunol. Meth. 1977, v. 16, p. 156-158.
  16. Прозоровский С.В., Раковская И.В., Вульфович Ю.В. Медицинская микоплазмология. М., 1995.

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