Mokronosova M.A.1, Turlapova E.V.1, Glushakova A.M.1, Pampura A.N.2, Zheltikova T.M.1
1 Mechnikov’s Research Institute for Vaccines and Sera, Moscow, Russia
2 Institute of Pediatrics, Moscow, Russia
Key words: IgE-antibodies, Malassezia, atopic dermatitis
Background. The objective was to study the species diversity and the proportion of lipophilic yeasts of the genus
Malassezia on the skin of children with atopic dermatitis and control groups, to evaluate age-related features
and clinical significance of sensitization to Malassezia and IgE-antibodies to Mn-SOD in children with atopic
Materials and methods. The study involved 144 children aged between 1 –14 years: 93 patients with atopic dermatitis,
a control group of K1 – 24 children with asthma and /or AR without atopic dermatitis and K2 – 27 healthy
children without skin diseases. The yeast Malassezia spp. identified by morphological and physiological characteristics
and analysis of the nucleotide sequences of the D1/D2 region of 26S (LSU) rDNA. For detection of IgE
antibodies to various allergens, including Malassezia, used the device and reagents ImmunoCap (Thermofisher,
Sweden); IgE-antibodies to Mn-SOD was detected by the method of ISAC ImmunoCap (Thermofisher, Sweden).
Results. Identified 5 species of Malassezia, absolutely dominated M. sympodialis. IgE-antibodies to Malassezia
spp. were detected in 45% (42/93) of patients with atopic dermatitis: 11% – children with ADL, 22% from the
ADB and 67% from the ADT. Statistically significant correlation between the level of IgE-antibodies to the yeast
Malassezia spp. and severity of atopic dermatitis (SCORAD) – r=0,5928, p<0,05 was revealed. The frequency
of detection and concentration of IgE-antibodies to Malassezia spp. is increasing with the age of the children.
Positive values of IgE-antibodies to Malassezia spp. were identified only in children with presentability. In 7 out
of 24 children (29%) with positive IgE values-aT to Malassezia spp. were identified IgE-antibodies to the major
fungal allergen – superoxide dismutase Mn (Mn-SOD).
Conclusion. Thus, neither species diversity nor the number of yeasts of the genus Malassezia spp. do not affect
the severity of atopic dermatitis. The clinical significance of IgE-antibodies to Malassezia spp. and IgE-antibodies
to Mn-SOD was shown.